[Expression of tumor suppressor gene PRDM1 in lung cancers].

Abstract

OBJECTIVE:

To determine the expression of tumor suppressor gene PRDM1 in lung cancers.

METHODS:

Forty-five cases were enrolled in this study, including squamous cell carcinoma (20 cases), adenocarcinoma (15 cases), and small cell cancer (10 cases). PRDM1 protein was detected in paraffin-embedded tissue by immunohistochemistry. Tumor cells in lung cancers were further selected by laser microdissection for RT-PCR analysis. PRDM1 protein in frozen tissue was also detected by Western blot.

RESULTS:

(1) PRDM1 protein was found in paraffin-embedded tissues in 90.0% (18/20) of squamous cell carcinoma, 13.3% (2/15) of adenocarcinoma, and 0 (0/10) small cell lung cancer. Squamous cell carcinoma predominantly expressed PRDM1 protein ( P < 0.01). (2) Gene product of PRDM1 DNA binding region was not found in microdissected tumor cells, but an abnormal PRDM1 protein about 70 KD was detected simultaneously in whole tumor tissue.

CONCLUSION:

PRDM1 may be considered as a specific biomarker in pulmonary squamous cell carcinoma. The abnormal PRDM1 expression both at transcriptional and protein levels indicated that this tumor suppressor gene lost its function, which may become a new target in the strategy of treatment for lung cancers.

• Authors: Yan-Yan Liu, Shu-Jun Yang, Jie Ma, Li Wang, Yuan-Hua Liu, Sai-Juan Chen, Zhi-Xiang Shen, Wei-Li Zhao
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 29 Issue 6 Pg. 434-6 (Jun 2007) ISSN: 0253-3766 [Print] China
• PMID: 17974277 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Repressor Proteins
• Transcription Factors
• PRDM1 protein, human
• Positive Regulatory Domain I-Binding Factor 1

Topics

• Adenocarcinoma (genetics, metabolism)
• Adult
• Aged
• Biomarkers, Tumor (genetics, metabolism)
• Blotting, Western
• Carcinoma, Small Cell (genetics, metabolism)
• Carcinoma, Squamous Cell (genetics, metabolism)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Lung Neoplasms (genetics, metabolism)
• Male
• Middle Aged
• Positive Regulatory Domain I-Binding Factor 1
• Repressor Proteins (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Transcription Factors (genetics, metabolism)