Ezrin and alpha-smooth muscle actin are immunohistochemical prognostic markers in conventional osteosarcomas.

Abstract

Ezrin is a cytoskeleton linker protein that is actively involved in the metastatic process of cancer cells. We have searched for a prognostic value of ezrin and some of its partners: alpha-smooth muscle actin and CD44H in 37 patients with an osteosarcoma. Automate immunohistochemistry (IHC) with anti-ezrin, alpha-smooth muscle actin and CD44H antibodies was performed in 66 specimens: 37 biopsies before chemotherapy, 16 resected tumours of "poor" responders and 13 metastases. The messenger RNA (mRNA) levels of ezrin of 13 frozen biopsies and 4 metastases were evaluated by real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). All results were correlated to the following clinical data. Ezrin expression by IHC was found in 62% of 37 biopsies in the different histological subtypes. A good correlation was found between positive or negative samples by IHC and mRNA levels. Ezrin expression was recorded in 84.5% of metastastic samples. The mean expression of ezrin was higher in metastases than biopsies (p = 0.024). In multivariate analysis, ezrin was an independent prognostic marker for event-free survival and overall survival (OS) with p < 0.001 and p = 0.003, respectively, and alpha-smooth muscle actin for OS only (p = 0.024). Our findings suggest that ezrin and alpha-smooth muscle actin are predictive IHC prognostic markers for patients with an osteosarcoma.

• Authors: Sébastien Salas, Catherine Bartoli, Jean-Laurent Deville, Jean Gaudart, Fréderic Fina, Arlette Calisti, Gérard Bollini, Georges Curvale, Jean-Claude Gentet, Florence Duffaud, Dominique Figarella-Branger, Corinne Bouvier
• Journal: Virchows Archiv : an international journal of pathology (Virchows Arch) Vol. 451 Issue 6 Pg. 999-1007 (Dec 2007) ISSN: 0945-6317 [Print] Germany
• PMID: 17786474 (Publication Type: Journal Article)

Chemical References

• Actins
• Biomarkers, Tumor
• Cytoskeletal Proteins
• RNA, Messenger
• ezrin

Topics

• Actins (genetics, metabolism)
• Biomarkers, Tumor (metabolism)
• Bone Neoplasms (metabolism, mortality, pathology, therapy)
• Combined Modality Therapy
• Cytoskeletal Proteins (genetics, metabolism)
• Female
• France (epidemiology)
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Muscle, Smooth (metabolism)
• Neoplasm Metastasis (pathology)
• Osteosarcoma (metabolism, mortality, secondary, therapy)
• RNA, Messenger (metabolism)
• Survival Rate