[Effect of synthesized polypeptide (P16) on inhibiting cell transdifferentiation and fibrosis induced by connective tissue growth factor].

Abstract

OBJECTIVE:

To explore the possibility of a CTGF originated hexadeca-peptide (named P16) to compete with the CTGF in binding integrin avP3 on rat tubular epithelial cells (NRK-52E) and inhibit the transdifferentiation and myofibroblasts of NRK-52E cells induced by CTGF.

METHODS:

The NRK-52E cells were cultured in a condition with the existence of CTGF, P16-FITC (P16 labeled with fluorescein isothiocyanate), or both for 24h. The immunofluorescence staining and RT-PCR were employed to detect the expressions of the protein and mRNA of alpha-SMA and the collagen I and IV which indicate the cell trans-differentiation and fibrosis.

RESULTS:

The P16 had stronger affinity with the NRK-52E cells than the CTGF. In a CTGF and P16 co-culture system, the P16 inhibited the expression of a-SMA, collagen I and IV up-regulated by the CTGF. However, P16 alone had no effect on cell trans-differentiation and fibrosis.

CONCLUSION:

The synthesized P16 is capable of binding with NRK-52E cells and inhibiting trans-differentiation and fibrosis of the NRK-52E cells induced by CTGF in vitro. This finding offers a possibility of developing a novel antifibrosis therapy that targets CTGF receptor.

• Authors: Wei Wang, Yu-jun Shi, Li Zhang, Qing Li, Jing Qiu, Zhi-dan Tu, Feng Ye, Hong Bu
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 38 Issue 4 Pg. 590-4 (Jul 2007) ISSN: 1672-173X [Print] China
• PMID: 17718418 (Publication Type: Journal Article)

Chemical References

• Actins
• CCN2 protein, rat
• Integrin alphaVbeta3
• Oligopeptides
• RNA, Messenger
• Connective Tissue Growth Factor
• Collagen
• Fluorescein-5-isothiocyanate

Topics

• Actins (genetics)
• Amino Acid Sequence
• Animals
• Binding, Competitive
• Cell Line
• Cell Transdifferentiation (drug effects)
• Collagen (genetics)
• Connective Tissue Growth Factor (chemistry, metabolism, pharmacology)
• Epithelial Cells (cytology, drug effects, metabolism, pathology)
• Fibrosis (chemically induced, metabolism)
• Fluorescein-5-isothiocyanate (metabolism)
• Integrin alphaVbeta3 (metabolism)
• Kidney Tubules (cytology)
• Molecular Sequence Data
• Oligopeptides (biosynthesis, chemistry, metabolism, pharmacology)
• RNA, Messenger (genetics, metabolism)
• Rats
• Up-Regulation (drug effects)