Differential expression of Candida albicans secreted aspartyl proteinase in human vulvovaginal candidiasis.

Abstract

The opportunistic pathogen Candida albicans secretes aspartic proteinases (Saps), which raises a particular interest because of their role as virulence factors. Candida albicans possesses at least 10 members of a SAP gene family, all of which have been sequenced. Although the expression of the SAP genes has been extensively characterised under laboratory growth conditions and Saps contribute to the virulence of C. albicans in animal models of infection, few studies have analysed the difference of the in vivo expression of these proteinases in recurrent vulvovaginal candidiasis (RVVC), vulvovaginal candidiasis (VVC) and asymptomatic Candida carriers. The RT-PCR protocol was used to determine which of the SAP 1 to SAP 7 genes are expressed by C. albicans during asymptomatic Candida carriage, VVC and RVVC infection in this study. We found SAP 2, the SAP 4-6 and SAP 7 were the predominant proteinase genes expressed in the sample of both Candida carriers and patients with VVC and RVVC. SAP 2 and SAP 4-6 mRNA were detected in all subjects. SAP 1 and SAP 3 transcripts were observed only in patients with VVC and RVVC. SAP 7 mRNA expressions were detected in several of the patients and carriers samples. All seven SAP genes were simultaneously expressed in some patients with VVC and RVVC. This study demonstrates the differential expression of the secreted aspartic proteinases (Saps) genes during colonisation and VVC and RVVC infection in humans and correlates the expression of specific Candida species virulence genes with active disease and anatomical location.

• Authors: Cui Hong Lian, Wei Da Liu
• Journal: Mycoses (Mycoses) Vol. 50 Issue 5 Pg. 383-90 (Sep 2007) ISSN: 0933-7407 [Print] Germany
• PMID: 17714358 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• RNA, Fungal
• RNA, Messenger
• Aspartic Acid Endopeptidases

Topics

• Aspartic Acid Endopeptidases (genetics, metabolism)
• Candida albicans (enzymology, genetics, pathogenicity)
• Candidiasis, Vulvovaginal (microbiology)
• Carrier State (microbiology)
• DNA Primers (genetics)
• Female
• Gene Expression (genetics)
• Gene Expression Profiling
• Genes, Fungal
• Humans
• Multigene Family
• RNA, Fungal (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Recurrence
• Reverse Transcriptase Polymerase Chain Reaction
• Sensitivity and Specificity
• Vagina
• Virulence