Abstract |
Barrier to autointegration factor (BAF) binds double-stranded DNA, selected histones, transcription regulators, lamins, and LAP2-emerin-MAN1 (LEM) domain proteins. During early Caenorhabditis elegans embryogenesis, BAF-1 is required to organize chromatin, capture segregated chromosomes within the nascent nuclear envelope, and assemble lamin and LEM domain proteins in reforming nuclei. In this study, we used C. elegans with a homozygous deletion of the baf-1 gene, which survives embryogenesis and larval stages, to report that BAF-1 regulates maturation and survival of the germline, cell migration, vulva formation, and the timing of seam cell fusion. In the seam cells, BAF-1 represses the expression of the EFF-1 fusogen protein, but fusion still occurs in C. elegans lacking both baf-1 and eff-1. This suggests the existence of an eff-1-independent mechanism for cell fusion. BAF-1 is also required to maintain the integrity of specific body wall muscles in adult animals, directly implicating BAF in the mechanism of human muscular dystrophies ( laminopathies) caused by mutations in the BAF- binding proteins emerin and lamin A.
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Authors | Ayelet Margalit, Esther Neufeld, Naomi Feinstein, Katherine L Wilson, Benjamin Podbilewicz, Yosef Gruenbaum |
Journal | The Journal of cell biology
(J Cell Biol)
Vol. 178
Issue 4
Pg. 661-73
(Aug 13 2007)
ISSN: 0021-9525 [Print] United States |
PMID | 17698609
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- BAF-1 protein, C elegans
- Caenorhabditis elegans Proteins
- Carrier Proteins
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Topics |
- Animals
- Caenorhabditis elegans
(embryology, metabolism)
- Caenorhabditis elegans Proteins
(genetics, metabolism)
- Carrier Proteins
(genetics, metabolism)
- Cell Movement
- Epidermal Cells
- Epidermis
(metabolism)
- Humans
- Muscles
(metabolism)
- Muscular Dystrophy, Emery-Dreifuss
(metabolism)
- Nuclear Envelope
(metabolism)
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