[Study on the changes of soluble intercellular adhesion molecule-1 level in sera of rats infected with Pneumocystis carinii].

Abstract

OBJECTIVES:

To examine sICAM-1 in Pneumocystis carinii pneumonia (PCP) and the effect of TMP-SMZ therapy on its level and on pathological and immunological changes in rats.

METHODS:

50 female Wistar rats were randomly divided into normal group (N group), PCP model group (PCP group) and TMP-SMZ therapy group (SMZ group). 1 mg of dexamethasone was injected intramuscularly twice a week for rats in PCP and SMZ groups to induce PCP. Normal saline was injected for N group in the same way. When the infection was confirmed, TMP-SMZ was given to rats in SMZ group by 25 mg/(kg.d) for 5 days for 3 courses with an interval of 2 weeks. sICAM-1 in serum was detected by ELISA, and the pathological changes in lungs and liver and the Pc in alveoli of lungs were observed.

RESULTS:

The level of sICAM-1 in PCP and SMZ groups at the 3rd week [(1.847+/-0.50) ng/ml, (1.787+/-0.59) ng/ml] was lower notably than that at 0 week [(2.407+/-0.81) ng/ml, [(2.478+/-0.59) ng/ml respectively] (P<0.05), and then increased gradually. It was significantly higher in PCP group at 9th week [(3.233+/-0.83) ng/ml] and at 12th week [(3.984+/-0.87) ng/ml] than that of 0 week (P<0.05). Its level in SMZ group at 12th week [(3.621+/-l.62) ng/ml] was also higher than that in 0 week [(2.478+/-0.59) ng/ml] (P<0.05). sICAM-1 level in both PCP and SMZ groups at 9th week and 12th week was higher than that of N group (P<0.05, P<0.01). There was no significant difference between SMZ and PCP groups at 9th week and 12th week (P>0.05).

CONCLUSION:

The sICAM-1 level in rats was low but significantly increases after the induction of PCP.

• Authors: Ke-Qin Liu, Wei-Dong Yin, Gui-Ping Xue, Jin-Shun Zhang
• Journal: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi) Vol. 25 Issue 1 Pg. 27-31, 35 (Feb 28 2007) ISSN: 1000-7423 [Print] China
• PMID: 17639695 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Intercellular Adhesion Molecule-1
• Trimethoprim, Sulfamethoxazole Drug Combination

Topics

• Animals
• Enzyme-Linked Immunosorbent Assay
• Female
• Host-Pathogen Interactions
• Intercellular Adhesion Molecule-1 (blood)
• Pneumocystis carinii (drug effects, physiology)
• Pneumonia, Pneumocystis (blood, drug therapy, microbiology)
• Random Allocation
• Rats
• Rats, Wistar
• Solubility
• Trimethoprim, Sulfamethoxazole Drug Combination (therapeutic use)