Abstract |
An extremely halophilic archaeon belonging to the order Halobacteriales was isolated from Fuente de Piedra salt lake, Spain. This strain, designated FP1(T), was a pleomorphic coccoid, neutrophilic and required at least 15 % (w/v) NaCl for growth. Strain FP1(T) grew at 37-60 degrees C, with optimal growth at 50 degrees C. Mg(2+) was not required, but growth was observed with up to 10 % (w/v) MgSO(4). Polar lipid analysis revealed the presence of mannose-6-sulfate(1-2)-glucose glycerol diether as a major glycolipid. Both C(20)C(20) and C(20)C(25) core lipids were present. The genomic DNA G+C content was 62.0 mol%. Phylogenetic analysis based on comparison of 16S rRNA gene sequences demonstrated that the isolate was most closely related to species of the genus Haloterrigena. DNA- DNA reassociation values between strain FP1(T) and the most closely related species of the genus Haloterrigena (Haloterrigena thermotolerans, Haloterrigena saccharevitans and Haloterrigena limicola) were lower than 29 %. It is therefore considered that strain FP1(T) represents a novel species of the genus Haloterrigena, for which the name Haloterrigena hispanica sp. nov. is proposed. The type strain is FP1(T) (=DSM 18328(T)=ATCC BAA-1310(T)).
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Authors | Ida Romano, Annarita Poli, Ilaria Finore, F Javier Huertas, Agata Gambacorta, Salvatore Pelliccione, Giancarlo Nicolaus, Licia Lama, Barbara Nicolaus |
Journal | International journal of systematic and evolutionary microbiology
(Int J Syst Evol Microbiol)
Vol. 57
Issue Pt 7
Pg. 1499-1503
(Jul 2007)
ISSN: 1466-5026 [Print] England |
PMID | 17625183
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Archaeal
- DNA, Ribosomal
- Glycolipids
- RNA, Archaeal
- RNA, Ribosomal, 16S
- Magnesium Chloride
- Sodium Chloride
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Topics |
- Base Composition
- DNA, Archaeal
(chemistry, isolation & purification)
- DNA, Ribosomal
(chemistry, isolation & purification)
- Genes, rRNA
- Glycolipids
(analysis)
- Halobacteriaceae
(chemistry, classification, isolation & purification, physiology)
- Magnesium Chloride
(metabolism)
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Phylogeny
- RNA, Archaeal
(genetics)
- RNA, Ribosomal, 16S
(genetics)
- Sequence Analysis, DNA
- Sequence Homology, Nucleic Acid
- Sodium Chloride
(metabolism)
- Spain
- Temperature
- Water Microbiology
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