Abstract | OBJECTIVE: METHODS: Peripheral blood mononuclear cells from 38 patients with RA were obtained before therapy and 2 weeks and 54 weeks after administration of the first dose of infliximab, and from 20 healthy control subjects. TNFalpha, TTP, TIA-1, and HuR gene expression levels were analyzed by real-time polymerase chain reaction. RESULTS: At baseline, TTP and HuR gene expression levels, as well as the TTP: TNFalpha, TTP:HuR, and TIA-1: TNFalpha gene expression ratios were lower in patients with RA than in control subjects, while expression of TNFalpha, TIA-1, and TIA-1:HuR was higher in patients with RA. The TTP:HuR expression ratio decreased significantly after administration of infliximab. Positive correlations were observed between TNFalpha and TTP, TNFalpha and TIA-1, TIA-1 and HuR, and TNFalpha and HuR gene expression in both healthy control subjects and patients with RA. At baseline, the TIA-1:HuR ratio tended to be higher in patients who achieved 50% improvement according to the American College of Rheumatology criteria (ACR50) at week 54 than in those who did not achieve at least an ACR20 response. CONCLUSION: Differences in ABP gene expression may affect TNFalpha gene expression. A higher TIA-1:HuR expression ratio might correlate with the response to infliximab therapy.
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Authors | Makoto Sugihara, Akito Tsutsumi, Eiji Suzuki, Ei Wakamatsu, Takeshi Suzuki, Hiroshi Ogishima, Taichi Hayashi, Yusuke Chino, Wataru Ishii, Mizuko Mamura, Daisuke Goto, Isao Matsumoto, Satoshi Ito, Takayuki Sumida |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 56
Issue 7
Pg. 2160-9
(Jul 2007)
ISSN: 0004-3591 [Print] United States |
PMID | 17599736
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Antibodies, Monoclonal
- Antigens, Surface
- ELAV Proteins
- ELAV-Like Protein 1
- ELAVL1 protein, human
- Poly(A)-Binding Proteins
- RNA-Binding Proteins
- T-Cell Intracellular Antigen-1
- TIA1 protein, human
- Tristetraprolin
- Tumor Necrosis Factor-alpha
- Infliximab
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Topics |
- Adult
- Anti-Inflammatory Agents
(therapeutic use)
- Antibodies, Monoclonal
(therapeutic use)
- Antigens, Surface
(genetics)
- Arthritis, Rheumatoid
(drug therapy, genetics, immunology)
- ELAV Proteins
- ELAV-Like Protein 1
- Female
- Gene Expression Regulation
(drug effects, immunology)
- Humans
- Infliximab
- Leukocytes, Mononuclear
(immunology)
- Male
- Middle Aged
- Poly(A)-Binding Proteins
(genetics)
- RNA-Binding Proteins
(genetics)
- Reference Values
- T-Cell Intracellular Antigen-1
- Tristetraprolin
(genetics)
- Tumor Necrosis Factor-alpha
(genetics)
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