High-level expression of fusion protein containing 10 tandem repeated GLP-1 analogs in yeast Pichia pastoris and its biological activity in a diabetic rat model.

Abstract

Glucagon-like peptide-1 (GLP-1), an incretin secreted by intestinal L-cells, can effectively lower blood glucose levels in patients with diabetes. A fusion gene, consisting of 10 tandem repeated GLP-1 analog genes, was expressed at a high level in the yeast Pichia pastoris. SDS polyacrylamide gel electrophoresis (SDS-PAGE), and Western Blotting results showed that fusion protein migrated as a single protein band with a molecular weight of 36 kDa. A biological activity test showed that the GLP-1 analog could significantly lower the level of serum glucose when GLP-1 purified analog was injected into diabetic rats. A potential strategy for large-scale production of fusion protein containing the 10 GLP-1 analogs as discovered, and a single GLP-1 analog was obtained from fusion protein digested with trypsin. This should be inspired foreign expression of medicinal short peptides and be valuable in further research on GLP-1 analog drugs in the treatment of diabetes mellitus.

• Authors: Jianhua Hou, Ruixiang Yan, Liquan Yang, Zhiqiang Wu, Cuiyan Wang, Dongfeng Ding, Na Li, Chunxiao Ma, Minggang Li
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 71 Issue 6 Pg. 1462-9 (Jun 2007) ISSN: 0916-8451 [Print] England
• PMID: 17587676 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• Recombinant Fusion Proteins
• Glucagon-Like Peptide 1

Topics

• Animals
• Blood Glucose (drug effects)
• Cloning, Molecular (methods)
• Diabetes Mellitus (drug therapy)
• Disease Models, Animal
• Glucagon-Like Peptide 1 (analogs & derivatives, genetics, therapeutic use)
• Pichia (genetics)
• Rats
• Recombinant Fusion Proteins (genetics, therapeutic use)
• Tandem Repeat Sequences