Overexpression of fucosyltransferase IV in A431 cell line increases cell proliferation.

Abstract	Fucosyltransferase IV is an essential enzyme that catalyzes the synthesis of fucosylated oligosaccharides by transferring GDP-fucose to the terminal N-acetylglucosamine with the alpha1,3-linkage. Lewis Y oligosaccharide has a terminal alpha1,3-linked fucose residue and elevation of Lewis Y level is seen in many epithelial cancers. The mechanism of Lewis Y elevation in neoplastic cells is still largely unknown. To study the impact of fucosyltransferase IV on Lewis Y expression and its role on neoplastic cell proliferation, a pEGFP-N1-FUT4 recombinant plasmid was developed and stably transfected into A431 cells. We found that fucosyltransferase IV overexpression promoted cell proliferation and increased the expression of proliferating cell nuclear antigen that correlated with Lewis Y augmentation. Cell cycle analysis demonstrated that fucosyltransferase IV overexpression facilitated cell cycle progression. In conclusion, fucosyltransferase IV overexpression augments Lewis Y expression to trigger neoplastic cell proliferation. These studies suggest that fucosyltransferase IV may serve as a potential therapeutic target for the treatment of Lewis Y-positive epithelial cancers.
Authors	Xuesong Yang, Zhenbo Zhang, Shuang Jia, Yuejian Liu, Xiaoqi Wang, Qiu Yan
Journal	The international journal of biochemistry & cell biology (Int J Biochem Cell Biol) Vol. 39 Issue 9 Pg. 1722-30 ( 2007) ISSN: 1357-2725 [Print] Netherlands
PMID	17556010 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	DNA, Complementary Lewis Blood Group Antigens Lewis X Antigen Lewis Y antigen Proliferating Cell Nuclear Antigen RNA, Messenger FUT4 protein, human Fucosyltransferases
Topics	Cell Cycle Cell Line, Tumor Cell Proliferation DNA, Complementary Fucosyltransferases (genetics, metabolism) Gene Expression Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans Lewis Blood Group Antigens (biosynthesis) Lewis X Antigen Proliferating Cell Nuclear Antigen (metabolism) RNA, Messenger (genetics, metabolism) Transfection

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