The late phase of ischemic preconditioning induces a prosurvival genetic program that results in marked attenuation of apoptosis.

Abstract

Although the cardioprotection afforded by the late phase of ischemic preconditioning (PC) in ischemia/reperfusion (I/R) injury has been well studied, it is unknown whether this beneficial effect can be attributed to inhibition of apoptosis. We hypothesized that ischemic PC affords protection by suppressing apoptosis and examined the underlying mechanisms. Myocardial infarction was produced in mice (30-min coronary occlusion). In animals preconditioned 24 h earlier with six 4-min coronary occlusion/4-min reperfusion (O/R) cycles, there was a marked decrease in apoptosis as assessed by three different parameters: hairpin-1 assay, caspase-3 activity, and immunohistochemical analysis of active caspase-3 and cleaved poly (ADP-ribose) polymerase-1 (PARP-1). This protective effect was accompanied by increased expression of multiple antiapoptotic proteins that regulate both the mitochondria-mediated (Bcl-x(L) and Mcl-1) and the death-receptor-mediated (c-FLIP(L) and c-FLIP(S)) pathway of apoptosis and by decreased expression of the proapoptotic protein Bad. This is the first demonstration that the late phase of ischemic PC attenuates cardiac apoptosis after ischemia/reperfusion injury and that this salubrious effect is associated with a complex genetic prosurvival program that results in modulation of several key proteins involved in both the mitochondrial and the death receptor pathways of apoptosis.

• Authors: Adam B Stein, Roberto Bolli, Yiru Guo, Ou-Li Wang, Wei Tan, Wen-Jian Wu, Xiaoping Zhu, Yanqing Zhu, Yu-Ting Xuan
• Journal: Journal of molecular and cellular cardiology (J Mol Cell Cardiol) Vol. 42 Issue 6 Pg. 1075-85 (Jun 2007) ISSN: 0022-2828 [Print] England
• PMID: 17490677 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Bad protein, mouse
• Bax protein, mouse
• Bcl2l1 protein, mouse
• CASP8 and FADD-Like Apoptosis Regulating Protein
• Cflar protein, mouse
• Mcl1 protein, mouse
• Myeloid Cell Leukemia Sequence 1 Protein
• Neoplasm Proteins
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• bcl-Associated Death Protein
• bcl-X Protein
• Parp1 protein, mouse
• Poly (ADP-Ribose) Polymerase-1
• Poly(ADP-ribose) Polymerases
• Caspase 3

Topics

• Animals
• Apoptosis (physiology)
• CASP8 and FADD-Like Apoptosis Regulating Protein (genetics, metabolism)
• Caspase 3 (analysis, metabolism)
• Enzyme Activation
• Immunohistochemistry
• Ischemic Preconditioning
• Male
• Mice
• Mice, Inbred ICR
• Myeloid Cell Leukemia Sequence 1 Protein
• Neoplasm Proteins (genetics, metabolism)
• Poly (ADP-Ribose) Polymerase-1
• Poly(ADP-ribose) Polymerases (metabolism)
• Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
• Time Factors
• bcl-2-Associated X Protein (genetics, metabolism)
• bcl-Associated Death Protein (genetics, metabolism)
• bcl-X Protein (genetics, metabolism)