[Effect of ketamine on endotoxin-induced septic shock in rats and its mechanism].

Abstract

OBJECTIVE:

To explore the effect of ketamine on septic shock in rat and its mechanism.

METHODS:

Sixty SD rats were randomly divided into control group, model group and ketamine group. Septic shock was replicated by intraperitoneal injection of 20 mg/kg lipopolysaccharide (LPS). Control group was given normal saline only. In ketamine group, the rats received intraperitoneal injection of 80 mg/kg ketamine 20 minutes before shock and 1 hour (ketamine 40 mg/kg) after shock. The survival time and survival rate were observed in each group. Serum tumor necrosis factor-alpha (TNF-alpha), myocardiac cyclic adenosine monophosphate (cAMP) were determined with radioimmunology assay, and expression of heat shock protein 70 (HSP70) was assessed by immunohistochemistry method.

RESULTS:

The survival rate was lower in model group (0) compared with control group (100%) and ketamine group (70%), the differences were significant (both P<0.05). TNF-alpha was higher, while cAMP was lower in model group compared with control group and ketamine group (all P<0.01). Positive expression of HSP70 in model group was lower than control group, while higher than ketamine group (both P<0.01). There were no significant differences in TNF-alpha and cAMP between control group and ketamine group (both P>0.05).

CONCLUSION:

Serum TNF-alpha is increased and myocardiac cAMP is decreased in LPS-induced septic shock, whereas ketamine can inhibit the effect of LPS and protect myocardium against sepsis probably by stimulating the expression of myocardiac HSP70.

• Authors: Hong Xiao, Hong-wei Xu, Hui Liu, Lan Zhang
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 19 Issue 5 Pg. 303-5 (May 2007) ISSN: 1003-0603 [Print] China
• PMID: 17490574 (Publication Type: English Abstract, Journal Article)

Chemical References

• Anesthetics, Dissociative
• HSP70 Heat-Shock Proteins
• Tumor Necrosis Factor-alpha
• Ketamine
• Cyclic AMP

Topics

• Anesthetics, Dissociative (therapeutic use)
• Animals
• Cyclic AMP (metabolism)
• Disease Models, Animal
• Female
• HSP70 Heat-Shock Proteins (metabolism)
• Ketamine (therapeutic use)
• Male
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Shock, Septic (drug therapy, metabolism)
• Tumor Necrosis Factor-alpha (metabolism)