Abstract | PURPOSE:
Carboxylesterase 2 (CES2) is involved in the activation of the anticancer drug irinotecan to its active metabolite SN-38. We previously identified a single nucleotide polymorphism (SNP), with an allele frequency around 10%, as possibly involved in enzyme expression (Clin Pharmacol Ther 76:528-535, 2004), which could explain the large individual variation in SN-38 disposition. METHODS: The 830C>G SNP, located in the 5' untranslated region of the gene, was analysed in various DNA samples extracted from: (1) the National Cancer Institute NCI-60 panel of human tumour cell lines; (2) a collection of 104 samples of normal tissue from colorectal cancer patients; (3) blood samples from a population of 95 normal subjects; (4) a collection of 285 human livers. CES2 genotypes were tentatively related to irinotecan cytotoxicity and CES2 expression in the NCI-60 panel; to response to treatment and event-free survival in colorectal cancer patients; and to CES2 expression and catalytic activity in subsets of the human liver collection. RESULTS: No significant relationship was found in the NCI-60 panel between CES2 830C>G genotype and irinotecan cytotoxicity or CES2 expression. No significant relationship was found between CES2 830C>G genotype and the toxicity and therapeutic efficacy (tumour response, event-free survival) of irinotecan in colorectal cancer patients. There was no significant relationship between CES2 830C>G genotype and CES2 expression and catalytic activity determined in a subset of genotype-selected liver samples. CONCLUSION: The 830C>G SNP of CES2 is unlikely to have significant functional consequences on CES2 expression, activity or function.
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Authors | Ricardo Bellott, Valérie Le Morvan, Virginie Charasson, Armelle Laurand, Marthe Colotte, Ulrich M Zanger, Kathrin Klein, Denis Smith, Jacques Bonnet, Jacques Robert |
Journal | Cancer chemotherapy and pharmacology
(Cancer Chemother Pharmacol)
Vol. 61
Issue 3
Pg. 481-8
(Mar 2008)
ISSN: 0344-5704 [Print] Germany |
PMID | 17483951
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Irinotecan
- DNA
- CES2 protein, human
- Carboxylesterase
- Camptothecin
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Topics |
- Alleles
- Amino Acid Substitution
- Antineoplastic Agents, Phytogenic
(pharmacokinetics)
- Camptothecin
(analogs & derivatives, pharmacokinetics)
- Carboxylesterase
(genetics)
- Cell Line, Tumor
- Colorectal Neoplasms
(drug therapy, genetics)
- DNA
(genetics)
- Genotype
- Humans
- Irinotecan
- Liver
(chemistry)
- Paraffin Embedding
- Polymorphism, Single Nucleotide
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Survival Analysis
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