Abstract | BACKGROUND:
Cholesterol elimination occurs through bile acid synthesis that starts within the liver from 7alpha-hydroxylation or in extrahepatic tissues from 27-hydroxylation. This study was aimed at investigating in vivo these two pathways in patients with chronic liver disease. METHODS: RESULTS: In patients with liver disease, the rate of plasma appearance of 7alpha-hydroxycholesterol was significantly reduced (1.44+/-0.96 vs. 2.75+/-1.43 mg/hour, p=0.03), the degree of reduction being related with the severity of the disease (p=0.01) whereas that of 27-hydroxycholesterol was unaffected. The rate of plasma appearance of 27-hydroxycholesterol was significantly related to its serum concentrations (r=0.54, p=0.03) and to its release from cultured macrophages ( r=0.85, p=0.03). CONCLUSIONS: In liver disease 7alpha-hydroxylation of cholesterol seems to be impaired while 27-hydroxylation is unaffected. Serum concentrations of 27-hydroxycholesterol are useful to obtain information on the activity of this alternative pathway.
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Authors | Andrea Crosignani, Marina Del Puppo, Matteo Longo, Emma De Fabiani, Donatella Caruso, Massimo Zuin, Mauro Podda, Norman B Javitt, Marzia Galli Kienle |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 382
Issue 1-2
Pg. 82-8
(Jul 2007)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 17482152
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bile Acids and Salts
- Hydroxycholesterols
- cholest-5-en-3 beta,7 alpha-diol
- 27-hydroxycholesterol
- Cholestanetriol 26-Monooxygenase
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Bile Acids and Salts
(biosynthesis, metabolism)
- Biosynthetic Pathways
(physiology)
- Case-Control Studies
- Cells, Cultured
- Cholestanetriol 26-Monooxygenase
(genetics, metabolism)
- Female
- Hepatitis, Chronic
(physiopathology)
- Humans
- Hydroxycholesterols
(blood, metabolism)
- Hydroxylation
- Liver Cirrhosis, Biliary
(physiopathology)
- Macrophages
(metabolism)
- Male
- Middle Aged
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