Abstract | BACKGROUND: METHODS: Male Sprague-Dawley rats received a single percutaneous injection of RTX (0.0005%, 0.1 mL) or vehicle at the sciatic nerve, and underwent surgery 3 h later to place four loose ligatures around the nerve on the side of drug administration. Responses to noxious heat (withdrawal latency, paw-lift duration), repetitive stimulation with von Frey filaments, and changes in hindpaw posture (toe spread, ventroflexion, and foot exorotation) were assessed. RESULTS: Perineural RTX administered before surgery completely prevented ligation-induced reduction in withdrawal latency, increase in paw lift duration and increase in withdrawal frequency to von Frey filaments. The preventive effect of RTX on the development of deficits in hindpaw posture was pronounced but not complete, e.g., on day 7 after surgery, the cumulative paw-posture score (0-6) was 1.69 +/- 0.92 with RTX and 4.06 +/- 1.68 with vehicle (P < 0.005). The effect of RTX used against the background of already developed neuropathy was limited to thermal hypoalgesia lasting for a relatively short period. CONCLUSION: Perineural RTX prevents the development of neuropathy caused by placing loosely constrictive ligatures on the sciatic nerve. Perioperative use of drugs acting via the TRPV1 receptors may hold the promise for preventing neuropathic pain after surgery on peripheral nerves.
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Authors | Igor Kissin, Cristina F Freitas, Edwin L Bradley Jr |
Journal | Anesthesia and analgesia
(Anesth Analg)
Vol. 104
Issue 5
Pg. 1210-6, tables of contents
(May 2007)
ISSN: 1526-7598 [Electronic] United States |
PMID | 17456676
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Diterpenes
- TRPV Cation Channels
- TRPV1 receptor
- resiniferatoxin
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Topics |
- Animals
- Diterpenes
(administration & dosage)
- Hyperalgesia
(drug therapy, physiopathology, prevention & control)
- Ligation
- Male
- Pain Measurement
(drug effects, methods)
- Peripheral Nerves
(drug effects, physiology)
- Rats
- Rats, Sprague-Dawley
- Sciatic Neuropathy
(drug therapy, physiopathology)
- TRPV Cation Channels
(physiology)
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