Relevance of combinatorial profiles of intermediate filaments and transcription factors for glioma histogenesis.

Abstract	In order to define specific markers for histogenesis of three well-characterized subgroups of human gliomas (pilocytic astrocytomas, glioblastoma multiforme and oligodendrogliomas), we studied the expression of relevant markers that characterize gliomagenesis, by immunohistochemistry and in situ hybridization. They include the intermediate filament proteins glial fibrillary acidic protein (GFAP), vimentin and nestin, the transcription factors Olig2, Nkx2.2 and Sox10, and the proteolipid protein transcripts plp/dm20. We show that the three major categories of human gliomas express a combinatorial profile of markers that gives new insights to their histogenesis and may help diagnosis. Pilocytic astrocytomas strongly express GFAP, vimentin, Olig2, Nkx2.2 and Sox10 but not nestin. In contrast, glioblastomas strongly express GFAP, vimentin and nestin but these tumours are heterogeneous regarding the expression of the transcription factors studied. Finally, in oligodendrogliomas, intermediate filament proteins are generally not observed whereas Olig2 was found in almost all tumour cells nuclei while only a subpopulation of tumour cells expressed Nkx2.2 and Sox10.
Authors	C Colin, I Virard, N Baeza, A Tchoghandjian, C Fernandez, C Bouvier, A Calisti, S Tong, P Durbec, D Figarella-Branger
Journal	Neuropathology and applied neurobiology (Neuropathol Appl Neurobiol) Vol. 33 Issue 4 Pg. 431-9 (Aug 2007) ISSN: 0305-1846 [Print] England
PMID	17442061 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Basic Helix-Loop-Helix Transcription Factors Biomarkers, Tumor DNA-Binding Proteins Glial Fibrillary Acidic Protein High Mobility Group Proteins Homeobox Protein Nkx-2.2 Homeodomain Proteins Intermediate Filament Proteins NES protein, human NKX2-2 protein, human Nerve Tissue Proteins Nestin Nuclear Proteins OLIG2 protein, human Oligodendrocyte Transcription Factor 2 SOX10 protein, human SOXE Transcription Factors Transcription Factors Vimentin Zebrafish Proteins nkx2.2b protein, zebrafish
Topics	Adult Aged Basic Helix-Loop-Helix Transcription Factors (genetics) Biomarkers, Tumor Brain Neoplasms (genetics) Child Child, Preschool DNA-Binding Proteins (genetics) Glial Fibrillary Acidic Protein (biosynthesis, genetics) Glioma (genetics) High Mobility Group Proteins (genetics) Homeobox Protein Nkx-2.2 Homeodomain Proteins (genetics) Humans Immunohistochemistry In Situ Hybridization Intermediate Filament Proteins (biosynthesis, genetics) Intermediate Filaments (genetics) Middle Aged Nerve Tissue Proteins (biosynthesis, genetics) Nestin Nuclear Proteins Oligodendrocyte Transcription Factor 2 SOXE Transcription Factors Transcription Factors (genetics) Vimentin (biosynthesis, genetics) Zebrafish Proteins

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