Immunohistochemical expression of RARalpha, RARbeta, and Cx43 in breast tumor cell lines after treatment with lycopene and correlation with RT-QPCR.

Abstract	Lycopene, the major carotenoid found in tomatoes, is a potent antioxidant associated with the prevention of degenerative diseases such as breast cancer. This effect could be due to the interaction between lycopene and retinoic acid receptors as well as the stimulation of gap junction communication and synthesis of connexin 43. The expression of the RARalpha, RARbeta, and Cx43 proteins was analyzed using immunohistochemistry in two breast cancer cell lines, MCF-7 and MDA-MB-231, and in a fibrocystic dystrophy cell line, MCF-10a, after a 48-hr exposure to 10 microM lycopene. A real-time quantitative PCR analysis was then performed to measure mRNA expression. RARalpha and Cx43 expression were increased at both mRNA and protein levels in two breast cell lines.
Authors	Nasséra Chalabi, Laetitia Delort, Samir Satih, Pierre Déchelotte, Yves-Jean Bignon, Dominique J Bernard-Gallon
Journal	The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (J Histochem Cytochem) Vol. 55 Issue 9 Pg. 877-83 (Sep 2007) ISSN: 0022-1554 [Print] United States
PMID	17438348 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Anticarcinogenic Agents Connexin 43 Protein Isoforms RARA protein, human RNA, Messenger Receptors, Retinoic Acid Retinoic Acid Receptor alpha retinoic acid receptor beta Carotenoids Lycopene
Topics	Anticarcinogenic Agents (pharmacology) Breast Neoplasms (pathology) Carotenoids (pharmacology) Cell Line Cell Line, Tumor Connexin 43 (biosynthesis, genetics) Female Fibrocystic Breast Disease (pathology) Humans Immunohistochemistry Lycopene Protein Isoforms (biosynthesis, genetics) RNA, Messenger (biosynthesis) Receptors, Retinoic Acid (biosynthesis, genetics) Retinoic Acid Receptor alpha Reverse Transcriptase Polymerase Chain Reaction Up-Regulation

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