Intra-articular injection of hyaluronate and indomethacin in rabbits with antigen-induced arthritis.

Abstract

Combined effects of hyaluronate and indomethacin in the treatment of rabbits with antigen-induced arthritis (AIA) were evaluated by assessing joint swelling, C-reactive protein (CRP) and prostaglandin E(2) (PGE(2)) levels with periodic intra-articular (ia) injections of hyaluronate alone (HA group) and with either a low or high concentration of indomethacin (LI-HA or HI-HA group). End-point analyses included matrix metalloproteinases-3 (MMP-3) activity and macroscopic and histological joint examinations. Results demonstrated that treatment in LI-HA and HI-HA groups resulted in statistically significant suppression of CRP, PGE(2, )and MMP-3 in comparison with those of HA group. Inhibition of serum CRP was only observed in LI-HA group. The order of serum MMP-3 inhibition was LI-HA>HI-HA>HA. Based on macroscopic and histological analyses of pannus formation, hyperplasia, inflammation, joint leakage and erosion, and loss of proteoglycan, the only statistically significant improvement was shown in LI-HA group compared to HA group and HI-HA group compared to control group.

• Authors: Yow-Jen Lo, Ming-Thau Sheu, Wen-Chi Tsai, Yun-Ho Lin, Jau-Le Li, Yu-Chih Liang, Chi-Ching Chang, Ming-Shium Hsieh, Chien-Ho Chen
• Journal: Rheumatology international (Rheumatol Int) Vol. 27 Issue 12 Pg. 1099-111 (Oct 2007) ISSN: 0172-8172 [Print] Germany
• PMID: 17436002 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Anti-Inflammatory Agents, Non-Steroidal
• Hyaluronic Acid
• C-Reactive Protein
• Matrix Metalloproteinase 3
• Dinoprostone
• Indomethacin

Topics

• Adjuvants, Immunologic (administration & dosage)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Arthritis, Experimental (blood, drug therapy, pathology)
• C-Reactive Protein (analysis)
• Dinoprostone (blood)
• Dose-Response Relationship, Drug
• Drug Therapy, Combination
• Hindlimb
• Hyaluronic Acid (administration & dosage)
• Indomethacin (pharmacology)
• Injections, Intra-Articular
• Joints (drug effects, enzymology, pathology)
• Matrix Metalloproteinase 3 (metabolism)
• Stifle (drug effects, enzymology, pathology)
• Synovial Membrane (drug effects, enzymology, pathology)
• Treatment Outcome