Carboxyl-terminal sequence variation of latent membrane protein 1 gene in Epstein-Barr virus-associated gastric carcinomas from Eastern China and Japan.

Abstract

OBJECTIVES:

To elucidate variations of latent membrane protein 1 (LMP1) in Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) and explore the LMP1 variations of neighboring countries, China and Japan.

METHODS:

In 12 and 8 EBVaGCs from eastern China and Japan, respectively, the C-termini of LMP1 were analyzed using PCR and sequencing. The sequences were compared with previously published strains and were characterized on a phylogenetic tree. The difference between Chinese and Japanese isolates was characterized.

RESULTS:

Ten of 12 Chinese GC isolates (83.3%) and all of the 8 (100%) Japanese GC isolates belonged to the China 1 strain. Also, B95-8 type isolates were found in 2 of 12 Chinese GC. In the 18 China 1 type isolates, additional mutations outside the signature sequence changes were found. All Japanese isolates (100%) had two or more additional mutations, whereas only 5 of 10 (50%) Chinese isolates had two or more additional mutations. The difference was statistically significant (p = 0.0359).

CONCLUSIONS:

China 1 is the dominant strain in GC from eastern China and Japan. The similarity to that of nasopharyngeal carcinoma (NPC) from China supports the view that China 1 strain represents a geographic-associated polymorphism rather than an NPC-associated polymorphism. Japanese isolates show more mutations than Chinese isolates, suggesting a geographic difference between Chinese and Japanese isolates in GC.

• Authors: Yun Wang, Kyosuke Kanai, Yukio Satoh, Bing Luo, Takeshi Sairenji
• Journal: Intervirology (Intervirology) Vol. 50 Issue 3 Pg. 229-36 ( 2007) ISSN: 0300-5526 [Print] Switzerland
• PMID: 17356301 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: (c) 2007 S. Karger AG, Basel.

Chemical References

• DNA, Viral
• EBV-associated membrane antigen, Epstein-Barr virus
• Viral Matrix Proteins

Topics

• China
• DNA, Viral (chemistry, genetics)
• Genotype
• Herpesvirus 4, Human (classification, genetics, isolation & purification)
• Humans
• Japan
• Molecular Epidemiology
• Mutation
• Phylogeny
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Protein Structure, Tertiary (genetics)
• Sequence Analysis, DNA
• Sequence Homology
• Stomach Neoplasms (virology)
• Viral Matrix Proteins (genetics)