High frequency of a retinoid X receptor gamma gene variant in familial combined hyperlipidemia that associates with atherogenic dyslipidemia.

Abstract

OBJECTIVE:

The genetic background of familial combined hyperlipidemia (FCHL) has not been fully clarified. Because several nuclear receptors play pivotal roles in lipid metabolism, we tested the hypothesis that genetic variants of nuclear receptors contribute to FCHL.

METHODS AND RESULTS:

We screened all the coding regions of the PPARalpha, PPARgamma2, PPARdelta, FXR, LXRalpha, and RXRgamma genes in 180 hyperlipidemic patients including 60 FCHL probands. Clinical characteristics of the identified variants were evaluated in other 175 patients suspected of coronary disease. We identified PPARalpha Asp140Asn and Gly395Glu, PPARgamma2 Pro12Ala, RXRgamma Gly14Ser, and FXR -1g->t variants. Only RXRgamma Ser14 was more frequent in FCHL (15%, P<0.05) than in other primary hyperlipidemia (4%) and in controls (5%). Among patients suspected of coronary disease, we identified 9 RXRgamma Ser14 carriers, who showed increased triglycerides (1.62+/-0.82 versus 1.91+/-0.42 [mean+/-SD] mmol/L, P<0.05), decreased HDL-cholesterol (1.32+/-0.41 versus 1.04+/-0.26, P<0.05), and decreased post-heparin plasma lipoprotein lipase protein levels (222+/-85 versus 149+/-38 ng/mL, P<0.01). In vitro, RXRgamma Ser14 showed significantly stronger repression of the lipoprotein lipase promoter than RXRgamma Gly14.

CONCLUSION:

These findings suggest that RXRgamma contributes to the genetic background of FCHL.

• Authors: Atsushi Nohara, Masa-aki Kawashiri, Thierry Claudel, Mihoko Mizuno, Masayuki Tsuchida, Mutsuko Takata, Shoji Katsuda, Kenji Miwa, Akihiro Inazu, Folkert Kuipers, Junji Kobayashi, Junji Koizumi, Masakazu Yamagishi, Hiroshi Mabuchi
• Journal: Arteriosclerosis, thrombosis, and vascular biology (Arterioscler Thromb Vasc Biol) Vol. 27 Issue 4 Pg. 923-8 (Apr 2007) ISSN: 1524-4636 [Electronic] United States
• PMID: 17272748 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cholesterol, LDL
• Lipids
• PPAR alpha
• Receptors, Cytoplasmic and Nuclear
• Retinoid X Receptor gamma
• Lipoprotein Lipase

Topics

• Adult
• Aged
• Animals
• COS Cells
• Chlorocebus aethiops
• Cholesterol, LDL (blood)
• Coronary Artery Disease (genetics)
• Dyslipidemias (genetics)
• Female
• Gene Frequency
• Genetic Variation
• Heterozygote
• Humans
• Hyperlipidemia, Familial Combined (blood, genetics)
• Lipids (blood)
• Lipoprotein Lipase (genetics)
• Male
• Middle Aged
• PPAR alpha (genetics)
• Promoter Regions, Genetic
• Receptors, Cytoplasmic and Nuclear (genetics)
• Retinoid X Receptor gamma (genetics)
• Transfection