Abstract | BACKGROUND: METHODS: H4 human neuroglioma cells stably transfected to express human full-length wild-type APP were exposed to 2% isoflurane for 6 hours. The cells were harvested at the end of the treatment. Caspase-3 activation was measured with quantitative Western blotting. RESULTS: We found that isoflurane induces cellular apoptosis in a dose-dependent manner, and that Congo red inhibits isoflurane-induced apoptosis in H4 human neuroglioma cells overexpressing APP. Interestingly, Congo red also inhibits staurosporine-induced apoptosis. CONCLUSION:
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Authors | Zhongcong Xie, Yuanlin Dong, Uta Maeda, Robert Moir, Sharon K Inouye, Deborah J Culley, Gregory Crosby, Rudolph E Tanzi |
Journal | The journals of gerontology. Series A, Biological sciences and medical sciences
(J Gerontol A Biol Sci Med Sci)
Vol. 61
Issue 12
Pg. 1300-6
(Dec 2006)
ISSN: 1079-5006 [Print] United States |
PMID | 17234824
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amyloid beta-Protein Precursor
- Anesthetics, Inhalation
- Isoflurane
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Topics |
- Amyloid beta-Protein Precursor
(analysis)
- Anesthetics, Inhalation
(adverse effects, pharmacology)
- Apoptosis
(drug effects)
- Blotting, Western
- Cells, Cultured
- Delirium
(pathology)
- Dementia
(pathology)
- Humans
- Isoflurane
(adverse effects, pharmacology)
- Neuroglia
(drug effects)
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