Abstract |
Immunotherapy and chemotherapy are generally effective against small tumors in animal models of cancer. However, these treatment regimens are generally ineffective against large, bulky tumors. We have found that a multimodality treatment regimen using DNA vaccination in combination with chemotherapeutic agent epigallocatechin-3-gallate (EGCG), a compound found in green tea, is effective in inhibiting large tumor growth. EGCG was found to induce tumor cellular apoptosis in a dose-dependent manner. The combination of EGCG and DNA vaccination led to an enhanced tumor-specific T-cell immune response and enhanced antitumor effects, resulting in a higher cure rate than either immunotherapy or EGCG alone. In addition, combined DNA vaccination and oral EGCG treatment provided long-term antitumor protection in cured mice. Cured animals rejected a challenge of E7-expressing tumors, such as TC-1 and B16E7, but not a challenge of B16 7 weeks after the combined treatment, showing antigen-specific immune responses. These results suggest that multimodality treatment strategies, such as combining immunotherapy with a tumor-killing cancer drug, may be a more effective anticancer strategy than single-modality treatments.
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Authors | Tae Heung Kang, Jin Hyup Lee, Chung Kil Song, Hee Dong Han, Byung Cheol Shin, Sara I Pai, Chien-Fu Hung, Cornelia Trimble, Jong-Seok Lim, Tae Woo Kim, T-C Wu |
Journal | Cancer research
(Cancer Res)
Vol. 67
Issue 2
Pg. 802-11
(Jan 15 2007)
ISSN: 0008-5472 [Print] United States |
PMID | 17234792
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Neoplasm
- Oncogene Proteins, Viral
- Papillomavirus E7 Proteins
- Vaccines, DNA
- oncogene protein E7, Human papillomavirus type 16
- Catechin
- epigallocatechin gallate
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Topics |
- Animals
- Antigens, Neoplasm
(immunology)
- Apoptosis
(drug effects, immunology)
- CD8-Positive T-Lymphocytes
(drug effects, immunology)
- Catechin
(analogs & derivatives, immunology, pharmacology)
- Combined Modality Therapy
- Dendritic Cells
(drug effects, immunology)
- Female
- Human papillomavirus 16
(immunology)
- Lymph Nodes
(immunology)
- Melanoma, Experimental
(drug therapy, immunology, therapy, virology)
- Mice
- Mice, Inbred C57BL
- Oncogene Proteins, Viral
(immunology)
- Papillomavirus E7 Proteins
- Th1 Cells
(drug effects, immunology)
- Vaccines, DNA
(immunology, pharmacology)
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