Abstract | BACKGROUND: Many breast, pancreatic, colonic and non-small-cell lung carcinoma lines express CEACAM6 (NCA-90) and CEACAM5 ( carcinoembryonic antigen, CEA), and antibodies to both can affect tumor cell growth in vitro and in vivo. Here, we compare both antigens as a function of histological phenotype in breast, pancreatic, lung, ovarian, and prostatic cancers, including patient-matched normal, primary tumor, and metastatic breast and colonic cancer specimens. METHODS:
Antigen expression was determined by immunohistochemistry (IHC) using tissue microarrays with MN-15 and MN-3 antibodies targeting the A1B1- and N-domains of CEACAM6, respectively, and the MN-14 antibody targeting the A3B3 domain of CEACAM5. IHC was performed using avidin- biotin-diaminobenzide staining. The average score +/- SD (0 = negative/8 = highest) for each histotype was recorded. RESULTS: CONCLUSION: CEACAM6 expression is elevated in many solid tumors, but variable as a function of histotype. Based on previous work demonstrating a role for CEACAM6 in tumor cell migration, invasion and adhesion, and formation of distant metastases (Blumenthal et al., Cancer Res 65: 8809-8817, 2005), it may be a promising target for antibody-based therapy.
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Authors | Rosalyn D Blumenthal, Evelyn Leon, Hans J Hansen, David M Goldenberg |
Journal | BMC cancer
(BMC Cancer)
Vol. 7
Pg. 2
(Jan 03 2007)
ISSN: 1471-2407 [Electronic] England |
PMID | 17201906
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Antigens, CD
- CEACAM6 protein, human
- Carcinoembryonic Antigen
- Cell Adhesion Molecules
- GPI-Linked Proteins
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Topics |
- Antigens, CD
(genetics)
- Carcinoembryonic Antigen
(genetics)
- Cell Adhesion Molecules
(genetics)
- GPI-Linked Proteins
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Neoplasm Metastasis
(genetics)
- Neoplasms
(genetics, mortality)
- Oligonucleotide Array Sequence Analysis
- Predictive Value of Tests
- Survival Analysis
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