Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe.

Abstract

An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive S. aureus and Gram-negative E. coli bacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.

• Authors: W Matthew Leevy, Seth T Gammon, Hua Jiang, James R Johnson, Dustin J Maxwell, Erin N Jackson, Manuel Marquez, David Piwnica-Worms, Bradley D Smith
• Journal: Journal of the American Chemical Society (J Am Chem Soc) Vol. 128 Issue 51 Pg. 16476-7 (Dec 27 2006) ISSN: 0002-7863 [Print] United States
• PMID: 17177377 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Fluorescent Dyes
• Molecular Probes
• Organometallic Compounds
• Picolines
• zinc(II) dipicolylamine

Topics

• Animals
• Disease Models, Animal
• Fluorescent Dyes (chemical synthesis, chemistry)
• Mice
• Molecular Probes (chemical synthesis, chemistry)
• Molecular Structure
• Organometallic Compounds (chemical synthesis, chemistry)
• Picolines (chemical synthesis, chemistry)
• Sensitivity and Specificity
• Spectroscopy, Near-Infrared (methods)
• Staphylococcal Infections (diagnosis)
• Staphylococcus aureus (chemistry)
• Tomography, Optical (methods)