[Expression profile of nasal NK/T cell lymphoma by cDNA microarray].

Abstract

OBJECTIVE:

To investigate the changes of gene expression profile in nasal NK/T cell lymphoma.

METHODS:

Total RNA was extracted from the fresh nasal NK/T cell lymphoma tissue and normal lymph node. Fluorescent labeled cDNA was obtained through synthesizing process by reverse transcription. After hybridization in the two identical microarrays consisting of 4096 genes, overexpressed or underexpressed tumor related genes were analyzed.

RESULTS:

In both experimental group and control group, there were six samples. A total of 365 (8.9%) genes was found to be differentially expressed by a factor of twofold or greater in both of two identical cDNA microarrays, which included oncogenes, tumor supressor genes, cell cycle regulators, apoptotic and antiapoptotic factors, DNA transcription factors, DNA repair and recombination factors, signal transduction genes, protein translation genes, as well as a large number of metabolic genes. Thirty-seven of these genes were found to be differentially expressed by a factor of fourfold or greater. The biochemical functions of these differentially expressed genes were diverse.

CONCLUSION:

This study demonstrates that many different kinds of genes are possibly involved in the initiation and progression of nasal NK/T lymphoma. cDNA microarray technique is useful in screening cancer gene expression for nasal NK/T lymphoma.

• Authors: Wei-Hu Wang, Ye-Xiong Li, Chen Lin, Xin-Fan Liu, Zi-Hao Yu, Yong-Wen Song, Xue-Yan Zhang, Ming Fu, Jing Jin, Shu-Lian Wang, Yue-Ping Liu
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 28 Issue 7 Pg. 518-22 (Jul 2006) ISSN: 0253-3766 [Print] China
• PMID: 17147117 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antigens, CD20
• CXCL9 protein, human
• Chemokine CXCL9
• Receptors, Immunologic
• Receptors, Natural Killer Cell

Topics

• Antigens, CD20 (genetics)
• Chemokine CXCL9 (genetics)
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• Humans
• Killer Cells, Natural (metabolism, pathology)
• Lymph Nodes (metabolism, pathology)
• Lymphoma, T-Cell (genetics, pathology)
• Nose Neoplasms (genetics, pathology)
• Oligonucleotide Array Sequence Analysis (methods)
• Receptors, Immunologic (genetics)
• Receptors, Natural Killer Cell