[Flow cytometric quantitative analysis of multiple tumor suppressor gene 1 (MTS1) and cyclooxygenase-2 (COX-2) gene expressions in invasive breast cancers and their clinical significance].

Abstract

OBJECTIVE:

The purpose of the present study was to explore the expression and clinical significance of multiple tumor suppressor gene 1 (MTS1) and cyclooxygenase-2 (COX-2) gene in invasive breast cancers.

METHODS:

Flow cytometry was used to analyze the expression level of MTS1 and COX-2 in cancer tissues and corresponding para-cancer tissues from 66 cases of primary invasive breast cancers.

RESULTS:

In breast cancer tissues, the expression of MTS1 and COX-2 assessed by relative fluorescence intensity were 0.84 and 10.54, respectively, and were 1.61 and 4.00 in corresponding para-cancer tissues, respectively. There was a significant difference between MTS1 and COX-2 expressions in cancer and corresponding para-cancer tissues (P <0.05). The differences of MTS1 and COX-2 expression of different ages, pathological types, tumor sizes or clinical stages of the breast cancer patients were not significant (P > 0.05). The MTS1 and COX-2 expressions were 1.12 and 5.94, respectively, in lymph node metastasis positive patients, and 0.79 and 13.05, respectively, in lymph node metastasis negative patients. The differences were significant (P <0.05).

CONCLUSION:

The preliminary research results suggest that MTS1 and COX-2 gene expressions play fairly important role in tumorigenesis and progression of breast cancers. MTS1 and COX-2 protein expressions have correlation with lymph node metastasis. This study provides theoretical basis for use of COX-2 selective inhibitors in prevention and treatment for breast cancer patients.

• Authors: Wen-shu Zuo, Ling Wei, Xing-Wu Wang, Yong-Sheng Wang, Zhi-Yong Yu, Ye Bi, Yan-Song Liu, Zheng-Bo Zhou
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 28 Issue 7 Pg. 515-7 (Jul 2006) ISSN: 0253-3766 [Print] China
• PMID: 17147116 (Publication Type: English Abstract, Journal Article)

Chemical References

• Biomarkers, Tumor
• Cyclin-Dependent Kinase Inhibitor p16
• Cyclooxygenase 2

Topics

• Adult
• Aged
• Biomarkers, Tumor (metabolism)
• Breast (metabolism, pathology)
• Breast Neoplasms (metabolism, pathology)
• Carcinoma, Ductal, Breast (metabolism, pathology)
• Cyclin-Dependent Kinase Inhibitor p16 (metabolism)
• Cyclooxygenase 2 (metabolism)
• Female
• Flow Cytometry
• Humans
• Lymphatic Metastasis
• Middle Aged
• Neoplasm Staging