Psoriasin (S100A7) is significantly up-regulated in human epithelial skin tumours.

Abstract

PURPOSE:

Psoriasin (S100A7) has originally been described to be expressed by psoriatic keratinocytes possibly as a result of altered differentiation and inflammation. As psoriasin was found to be overexpressed in human breast and bladder cancer suggesting a role in tumour progression, we investigated the expression of psoriasin in human epithelial skin tumours.

METHODS:

Realtime reverse transcription-polymerase chain reaction experiments were performed to analyse the mRNA-expression levels of psoriasin together with involucrin as a marker for epithelial differentiation and interleukin-8 (IL-8) as a marker for inflammation in skin biopsy samples from patients with precancerous skin lesions (PSL, n = 6), squamous cell carcinoma (SCC, n = 11), basal cell carcinoma (BCC, n = 17), and healthy controls (n = 10).

RESULTS:

Unexpectedly, mRNA expression levels for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) revealed a variation of up to 600-fold in all cDNA-samples under investigation, indicating that GAPDH is not suitable as a housekeeping gene in human skin samples. Psoriasin mRNA expression was significantly up-regulated in samples of PSL, SCC and BCC. In situ hybridisation and immunohistochemical examinations identified psoriasin mRNA and protein expression in the differentiated layers of the epidermis. IL-8 mRNA expression was significantly up-regulated in SCC, however, there was no correlation between elevated levels of psoriasin and the expression of IL-8.

CONCLUSION:

Similar to the findings in breast and bladder cancer, the up-regulation of psoriasin might play a role in the progression of skin cancer. The expression of psoriasin in human skin tumours seems to be independent from differentiation and inflammation.

• Authors: Noemi Moubayed, Michael Weichenthal, Jürgen Harder, Elke Wandel, Michael Sticherling, Regine Gläser
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 133 Issue 4 Pg. 253-61 (Apr 2007) ISSN: 0171-5216 [Print] Germany
• PMID: 17136347 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Calcium-Binding Proteins
• Interleukin-8
• RNA, Neoplasm
• S100 Calcium Binding Protein A7
• S100 Proteins
• S100A7 protein, human
• Glyceraldehyde-3-Phosphate Dehydrogenases

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (metabolism)
• Calcium-Binding Proteins (metabolism)
• Carcinoma, Basal Cell (genetics, metabolism, pathology)
• Carcinoma, Squamous Cell (genetics, metabolism, pathology)
• Child
• Female
• Gene Expression Regulation, Neoplastic
• Glyceraldehyde-3-Phosphate Dehydrogenases (genetics)
• Humans
• Immunohistochemistry
• In Situ Hybridization
• Interleukin-8 (metabolism)
• Male
• Middle Aged
• Precancerous Conditions (genetics, metabolism, pathology)
• RNA, Neoplasm (genetics, isolation & purification)
• Reverse Transcriptase Polymerase Chain Reaction
• S100 Calcium Binding Protein A7
• S100 Proteins
• Skin Neoplasms (genetics, metabolism, pathology)
• Up-Regulation