Abstract | OBJECTIVE: METHODS:
IgA, IgM, and IgG subclasses of anti-CCP were measured by enzyme-linked immunosorbent assay in serum samples that were obtained from IgG anti-CCP antibody-positive patients with UA (n = 110) and IgG anti-CCP antibody-positive patients with RA (n = 152) early after the onset of arthritis. Patients with UA in whom RA developed within 1 year (UA-->RA) were compared with patients with UA in whom RA did not develop within 1 year (UA-->UA). In addition, baseline serum samples obtained from a subset of patients with RA (n = 64) were compared with sera obtained from the same patients a median of 7 years later. RESULTS:
IgM anti-CCP was present in early samples from both patients with UA and patients with RA and in followup samples from patients with RA. Several IgG anti-CCP antibody-positive patients who did not have IgM anti-CCP early after disease onset did display IgM anti-CCP later in the course of the arthritis. A diverse pattern of isotype usage was detected in early samples, with a trend toward lower frequencies of all isotypes of anti-CCP in patients with UA compared with patients with RA and in UA-->UA patients compared with UA-->RA patients. Levels of all isotypes except IgG1 had decreased after 7 years. CONCLUSION: These data indicate development of the anti-CCP isotype repertoire into full usage early in the course of arthritis. The sustained presence of IgM anti-CCP indicates ongoing recruitment of new B cells into the anti-CCP response, reflecting a continuous (re)activation of the RA-specific anti-CCP response during the course of anti-CCP-positive arthritis.
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Authors | K N Verpoort, C M Jol-van der Zijde, E A M Papendrecht-van der Voort, A Ioan-Facsinay, J W Drijfhout, M J D van Tol, F C Breedveld, T W J Huizinga, R E M Toes |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 54
Issue 12
Pg. 3799-808
(Dec 2006)
ISSN: 0004-3591 [Print] United States |
PMID | 17133560
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Immunoglobulin Isotypes
- Peptides, Cyclic
- cyclic citrullinated peptide
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Topics |
- Antibody Formation
- Arthritis, Rheumatoid
(immunology)
- Autoantibodies
(immunology)
- B-Lymphocytes
(immunology)
- Humans
- Immunoglobulin Isotypes
(immunology)
- Peptides, Cyclic
(immunology)
- Time Factors
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