Elevated soluble ADAM8 in bronchoalveolar lavage fluid in patients with eosinophilic pneumonia.

Abstract

BACKGROUND:

ADAM (a disintegrin and metalloprotease) family members, characterized by a metalloprotease and a disintegrin domain, are membrane-anchored glycoproteins involved in proteolysis and cell adhesion. ADAM8 might have an important role in allergic inflammation. It can cleave a variety of substrates and is a sheddase for VCAM-1 and CD23, the low-affinity IgE receptors.

METHODS:

To evaluate the contribution of ADAM8 to the pathogenesis of eosinophilic pneumonia (EP), we measured the concentrations of soluble ADAM8 (sADAM8) and its substrates, soluble VCAM-1 (sVCAM-1) and soluble CD23 (sCD23), in bronchoalveolar lavage fluid from patients with smoking-induced acute eosinophilic pneumonia (AEP), chronic idiopathic eosinophilic pneumonia (CEP), and drug-induced eosinophilic pneumonia (drug-EP).

RESULTS:

The sADAM8 and sVCAM-1 concentrations were increased in AEP and CEP. The sCD23 concentration was elevated in AEP. In AEP, but not CEP, the sADAM8 concentration significantly correlated with those of both sVCAM and sCD23.

CONCLUSION:

The pathogenesis of AEP, CEP, and drug-EP was distinct with regard to ADAM8. Our results are the first to associate ADAM8 with eosinophilic responses and lung inflammation in humans.

• Authors: Osamu Matsuno, Eishi Miyazaki, Shinichi Nureki, Takuya Ueno, Masaru Ando, Kazunobu Ito, Toshihide Kumamoto, Yasunori Higuchi
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 142 Issue 4 Pg. 285-90 ( 2007) ISSN: 1018-2438 [Print] Switzerland
• PMID: 17124430 (Publication Type: Journal Article)

Chemical References

• CD3 Complex
• Membrane Proteins
• Vascular Cell Adhesion Molecule-1
• ADAM Proteins
• ADAM8 protein, human

Topics

• ADAM Proteins (immunology)
• Adolescent
• Adult
• Bronchoalveolar Lavage Fluid (immunology)
• CD3 Complex (immunology)
• Female
• Humans
• Male
• Membrane Proteins (immunology)
• Middle Aged
• Pulmonary Eosinophilia (immunology)
• Solubility
• Vascular Cell Adhesion Molecule-1 (immunology)