Abstract | BACKGROUND: METHODS: Biopsy specimens of eutopic endometrium were obtained from 22 patients with endometriosis and from 14 women that served as controls. Apoptosis was examined in EEC after incubation with LA and Antide. Bax, Bcl-2, Fas and FasL expression was evaluated after exposure to LA, Antide or a combination of both. The percentage of apoptotic cells (%ApC) was assessed by the acridine orange- ethidium bromide technique, and protein expression was evaluated by western blot and immunocytochemistry. RESULTS: LA 100 and 1000 ng/ml increased the %ApC in EEC from patients with endometriosis (both P < 0.05) and controls (p < 0.05 and P < 0.01, respectively). Antide 10(-5) M increased the %ApC in EEC from patients with endometriosis and controls (P < 0.01). In EEC from women with endometriosis, Bax expression increased after treatment with LA, Antide and LA + Antide (P < 0.05, P < 0.001 and P < 0.001), whereas Bcl-2 expression decreased after exposure to LA and Antide (P < 0.001 and P < 0.01). FasL expression increased after LA, Antide and LA + Antide treatments (P < 0.01, P < 0.001 and P < 0.01). No significant changes were observed on Fas expression. CONCLUSIONS:
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Authors | M Bilotas, R I Barañao, R Buquet, C Sueldo, M Tesone, G Meresman |
Journal | Human reproduction (Oxford, England)
(Hum Reprod)
Vol. 22
Issue 3
Pg. 644-53
(Mar 2007)
ISSN: 0268-1161 [Print] England |
PMID | 17092985
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- FAS protein, human
- Fas Ligand Protein
- Oligopeptides
- Proto-Oncogene Proteins c-bcl-2
- bcl-2-Associated X Protein
- fas Receptor
- Gonadotropin-Releasing Hormone
- iturelix
- Leuprolide
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Topics |
- Apoptosis
(drug effects)
- Cells, Cultured
- Endometriosis
(metabolism)
- Endometrium
(drug effects, metabolism)
- Epithelial Cells
(drug effects)
- Fas Ligand Protein
(biosynthesis)
- Female
- Gene Expression Regulation
(drug effects)
- Gonadotropin-Releasing Hormone
(agonists, antagonists & inhibitors)
- Humans
- Immunohistochemistry
- Infertility, Female
(physiopathology)
- Leuprolide
(pharmacology)
- Oligopeptides
(pharmacology)
- Proto-Oncogene Proteins c-bcl-2
(biosynthesis)
- bcl-2-Associated X Protein
(biosynthesis)
- fas Receptor
(biosynthesis)
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