[Inhibitory effect of weikangfu recipe on growth of mouse S180 tumor and its apoptotic induction].

Abstract

OBJECTIVE:

To study the growth inhibition of Weikangfu recipe on S180 tumor and its apoptotic induction.

METHOD:

S180-bearing mice were orally administrated with different dosages of Weikangfu recipe, and the growth inhibition was evaluated; apoptotic cells induced were detected by flow cytometry and DNA agarose gel electrophoresis.

RESULT:

Weikangfu recipe showed significant inhibition on the growth of S180 tumor in a dose-dependent manner, compared with the control group. From apoptotic analyses, Weikangfu recipe induced a dose-dependent apoptosis of S180 tumor cells and arrested the cell cycle distribution at G0-G1 phase. At the same time, the up-regulation of p53 and bax and down-regulation of bcl-2 were observed in S180 tumor cells of the treated groups.

CONCLUSION:

Our findings demonstrate that Weikangfu recipe can significantly inhibit the growth of S180 tumor and induce apoptosis through expression alteration of p53, bax and bcl-2.

• Authors: Xiao-Hua Nie, Bao-Jun Shi, Wen-Yi Tao
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 31 Issue 17 Pg. 1457-60 (Sep 2006) ISSN: 1001-5302 [Print] China
• PMID: 17087091 (Publication Type: English Abstract, Journal Article)

Chemical References

• Drug Combinations
• Drugs, Chinese Herbal
• Proto-Oncogene Proteins c-bcl-2
• Tumor Suppressor Protein p53
• bcl-2-Associated X Protein
• weikangfu

Topics

• Animals
• Apoptosis (drug effects)
• Cell Cycle (drug effects)
• Dose-Response Relationship, Drug
• Drug Combinations
• Drugs, Chinese Herbal (administration & dosage, isolation & purification, pharmacology)
• Female
• Male
• Mice
• Neoplasm Transplantation
• Plants, Medicinal (chemistry)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Random Allocation
• Sarcoma 180 (pathology)
• Tumor Suppressor Protein p53 (metabolism)
• bcl-2-Associated X Protein (metabolism)