Abstract | OBJECTIVE: The aim of the present study was to investigate collagen metabolism after anabolic and catabolic stimulation of chondrocytes ex vivo. DESIGN: RESULTS: In the bovine articular cartilage explants, IGF-I induced an increase in PIINP level up to 4.8+/-1.1[ng/ml]/mg cartilage whereas CTX-II remained below 0.1+/-0.1[ng/ml]/mg cartilage. In the catabolic stimulated explants both PIINP and CTX-II were released to the supernatant, reaching concentrations of 9.0+/-1.4 and 9.1+/-2.2[ng/ml]/mg cartilage, respectively. RA patients had significantly lower serum concentrations of PIINP (3.4+/-3.7 ng/ml) compared with those healthy individuals (18.7+/-12.41 ng/ml, P<0.001). In contrast, RA patients had significantly higher urinary CTX-II (0.8+/-0.8 mg/mmol) compared to the healthy controls (0.1+/-0.08 mg/mmol, P=0.004). CONCLUSIONS: This study is the first to demonstrate that precursors and degradation products of type II collagen released into the supernatant can effectively reflect the anabolic and catabolic activities of stimulated cartilage explants.
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Authors | A K Olsen, B C Sondergaard, I Byrjalsen, L B Tanko, C Christiansen, A Müller, G E Hein, M A Karsdal, P Qvist |
Journal | Osteoarthritis and cartilage
(Osteoarthritis Cartilage)
Vol. 15
Issue 3
Pg. 335-42
(Mar 2007)
ISSN: 1063-4584 [Print] England |
PMID | 17045814
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Collagen Type II
- Peptides
- Procollagen
- Tumor Necrosis Factor-alpha
- Insulin-Like Growth Factor I
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Topics |
- Animals
- Arthritis, Rheumatoid
(metabolism)
- Biomarkers
- Cartilage, Articular
(metabolism)
- Cattle
- Chondrocytes
(metabolism)
- Collagen Type II
(metabolism)
- Insulin-Like Growth Factor I
- Peptides
(metabolism)
- Procollagen
(metabolism)
- Tumor Necrosis Factor-alpha
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