Combination of surgical resection and photodynamic therapy of 9L gliosarcoma in the nude rat.

Abstract

The objective of the present study was to investigate the treatment of 9L gliosarcoma brain tumor in the rat with the combination of surgical resection and photodynamic therapy (PDT). Nude rats with intracranial 7-day-old 9L gliomas were randomly subjected to no treatment, PDT alone (Photofrin: 2 mg kg(-1), optical: 80 J cm(-2)), surgical resection alone or resection combined with 2 mg kg(-1) Photofrin-mediated PDT at an optical dose of 80 J cm(-2). All animals were sacrificed 14 days after tumor implantation. Hematoxylin-and-eosin and immunohistochemical stainings were performed to assess the tumor volume and the expression of vascular endothelial growth factor (VEGF) in the brain adjacent to the tumor (BAT) as well as the tumor cell apoptosis and proliferation. Our data show that both surgical resection alone and PDT alone significantly decreased tumor volume, but furthermore, surgical resection combined with PDT significantly reduced the tumor volume and reduced local tumor infiltration compared to either surgical resection or PDT treatment alone. PDT treatment with or without resection increased tumor apoptosis, but resection alone did not alter the tumor cell apoptosis compared with a nontreatment control group. Both surgical resection alone and PDT alone induced a significant increase in VEGF expression in the BAT; however intraoperative PDT did not further increase VEGF expression, compared with surgery alone or PDT alone. No significant differences were found in tumor cell proliferation as indicated by Ki67 immunoreactivity among the four groups. Our results suggest that PDT enhances the efficacy of surgical resection in the management of malignant gliomas without increasing VEGF expression in the BAT.

• Authors: Xuepeng Zhang, Feng Jiang, Steven N Kalkanis, Hongyan Yang, Zhenggang Zhang, Mark Katakowski, Xin Hong, Xuguang Zheng, Michael Chopp
• Journal: Photochemistry and photobiology (Photochem Photobiol) 2006 Nov-Dec Vol. 82 Issue 6 Pg. 1704-11 ISSN: 0031-8655 [Print] United States
• PMID: 17007560 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Dihematoporphyrin Ether

Topics

• Animals
• Brain (drug effects, pathology, radiation effects)
• Brain Neoplasms (drug therapy, surgery)
• Cell Line, Tumor
• Dihematoporphyrin Ether (therapeutic use)
• Gliosarcoma (drug therapy, surgery)
• Photochemotherapy
• Rats
• Rats, Nude