Abstract |
Lyme disease is the most common arthropod-borne disease in North America and Europe. At present, there is no commercially available vaccine for use in humans. Outer surface protein C ( OspC) has antigenic and expression characteristics that make it an attractive vaccine candidate; however, sequence heterogeneity has impeded its use as a vaccinogen. Sequence analyses have identified 21 well defined OspC phyletic groups or "types" (designated A-U). In this report we have mapped the linear epitopes presented by OspC types B, K, and D during human and murine infection and exploited these epitopes (along with the previously identified type A OspC linear epitopes) in the development of a recombinant, tetravalent, chimeric vaccinogen. The construct was found to be highly immunogenic in mice and the induced antibodies surface labeled in vitro cultivated spirochetes. Importantly, vaccination induced complement-dependent bactericidal antibodies against strains expressing each of the OspC types that were incorporated into the construct. These results suggest that an effective and broadly protective polyvalent OspC-based Lyme disease vaccine can be produced as a recombinant, chimeric protein.
|
Authors | Christopher G Earnhart, Eric L Buckles, Richard T Marconi |
Journal | Vaccine
(Vaccine)
Vol. 25
Issue 3
Pg. 466-80
(Jan 05 2007)
ISSN: 0264-410X [Print] Netherlands |
PMID | 16996663
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Epitopes
- Lyme Disease Vaccines
- Phosphatidylcholines
- Vaccines, Synthetic
- 1-oleoyl-2-stearoylphosphatidylcholine
|
Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Borrelia burgdorferi
(immunology, isolation & purification)
- Cloning, Molecular
- Enzyme-Linked Immunosorbent Assay
- Epitopes
(immunology)
- Fluorescent Antibody Technique
- Immunoblotting
- Lyme Disease
(immunology, prevention & control)
- Lyme Disease Vaccines
(genetics, immunology)
- Male
- Mice
- Mice, Inbred C3H
- Models, Molecular
- Molecular Sequence Data
- Phosphatidylcholines
(genetics, immunology)
- Reverse Transcriptase Polymerase Chain Reaction
- Species Specificity
- Vaccines, Synthetic
(genetics, immunology)
|