Reversible blockade of electron transport during ischemia protects mitochondria and decreases myocardial injury following reperfusion.

Abstract

Cardiac mitochondria sustain damage during ischemia and reperfusion, contributing to cell death. The reversible blockade of electron transport during ischemia with amobarbital, an inhibitor at the rotenone site of complex I, protects mitochondria against ischemic damage. Amobarbital treatment immediately before ischemia was used to test the hypothesis that damage to mitochondrial respiration occurs mainly during ischemia and that protection of mitochondria during ischemia leads to decreased cardiac injury with reperfusion. Langendorff-perfused Fischer-344 rat hearts were treated with amobarbital (2.5 mM) or vehicle for 1 min immediately before 25 min of global ischemia. Both groups were reperfused for 30 min without additional treatment. Subsarcolemmal (SSM) and interfibrillar (IFM) populations of mitochondria were isolated after reperfusion. Ischemia and reperfusion decreased state 3 and increased state 4 respiration rate in both SSM and IFM. Amobarbital treatment protected oxidative phosphorylation measured following reperfusion and improved the coupling of respiration. Cytochrome c content measured in SSM and IFM following reperfusion decreased in untreated, but not in amobarbital-treated, hearts. H(2)O(2) release from SSM and IFM isolated from amobarbital-treated hearts during reperfusion was markedly decreased. Amobarbital treatment before ischemia improved recovery of contractile function (percentage of preischemic developed pressure: untreated 51 +/- 4%, n = 12; amobarbital 70 +/- 4%, n = 11, p < 0.01) and substantially reduced infarct size (untreated 32 +/- 2%, n = 7; amobarbital 13 +/- 2%, n = 7, p < 0.01). Thus, mitochondrial damage occurs mainly during ischemia rather than during reperfusion. Reperfusion in the setting of preserved mitochondrial respiratory function attenuates the mitochondrial release of reactive oxygen species, enhances contractile recovery, and decreases myocardial infarct size.

• Authors: Qun Chen, Shadi Moghaddas, Charles L Hoppel, Edward J Lesnefsky
• Journal: The Journal of pharmacology and experimental therapeutics (J Pharmacol Exp Ther) Vol. 319 Issue 3 Pg. 1405-12 (Dec 2006) ISSN: 0022-3565 [Print] United States
• PMID: 16990510 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Cytochromes
• Cytochromes c
• Hydrogen Peroxide
• Citrate (si)-Synthase
• Amobarbital

Topics

• Amobarbital (therapeutic use)
• Animals
• Citrate (si)-Synthase (metabolism)
• Cytochromes (metabolism)
• Cytochromes c (metabolism)
• Electron Transport (drug effects)
• Hydrogen Peroxide (pharmacology)
• In Vitro Techniques
• Male
• Mitochondria, Heart (drug effects, pathology)
• Myocardial Contraction (drug effects)
• Myocardial Infarction (pathology, prevention & control)
• Myocardial Reperfusion Injury (pathology, prevention & control)
• Myocardium (pathology)
• Oxidative Phosphorylation (drug effects)
• Oxygen Consumption (drug effects)
• Rats
• Rats, Inbred F344
• Sarcolemma (drug effects, metabolism)