Comparison of the antioxidant status in tracheal and bronchoalveolar epithelial lining fluids in recurrent airway obstruction.

Abstract

REASONS FOR PERFORMING STUDY:

Following a period of airway inflammation the clearance of inflammatory cells along the mucociliary escalator may impose a considerable oxidant load on the trachea.

OBJECTIVES:

To determine the degree of oxidative stress in tracheal epithelial lining fluid (ELF) in comparison to that present in peripheral airways after an acute exposure to organic dust.

METHODS:

Tracheal wash fluid and bronchoalveolar lavage fluid (BALF) were collected for cytology and antioxidant analyses from 6 recurrent airway obstruction (RAO)-affected horses and 6 healthy control horses before and after stabling on straw bedding for 24 h.

RESULTS:

In RAO-affected horses, organic dust exposure resulted in a significant decrease in ascorbic acid concentration in tracheal ELF (P<0.0001), which was greater than the decrease in bronchoalveolar ELF (P = 0.0003). The percentage decrease in tracheal ELF ascorbic acid correlated with the percentage decrease in bronchoalveolar ELF ascorbic acid (r = 0.76; P = 0.004) following exposure.

CONCLUSIONS:

Acute organic dust exposure results in significant antioxidant depletion in the trachea, which may reflect inflammation and oxidative processes in peripheral airways.

POTENTIAL RELEVANCE:

Further work is required to evaluate the role of ascorbic acid depletion in the pathogenesis of RAO.

• Authors: C M Deaton, D J Marlin, L Deaton, N C Smith, P A Harris, R C Schroter, F J Kelly
• Journal: Equine veterinary journal (Equine Vet J) Vol. 38 Issue 5 Pg. 417-22 (Sep 2006) ISSN: 0425-1644 [Print] United States
• PMID: 16986601 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Dust
• Ascorbic Acid

Topics

• Animals
• Antioxidants (analysis, metabolism)
• Ascorbic Acid (analysis)
• Bronchoalveolar Lavage Fluid (chemistry, cytology)
• Dust
• Epithelium (metabolism, physiopathology)
• Female
• Horse Diseases (metabolism, physiopathology)
• Horses
• Lung Diseases, Obstructive (metabolism, physiopathology, veterinary)
• Male
• Oxidation-Reduction
• Oxidative Stress
• Recurrence
• Trachea (cytology, metabolism, pathology)