Yisheng injection decreases the expression of H60 and RAE-1 genes in ischemic mice liver.

Abstract

OBJECTIVE:

Major histocompatability complex class I chain-related antigen A, B (MICA, B) functions as ligands for human NKG2D receptors, which may play a role in graft rejection and cellular stress. In this study we explored the effect of ischemia/reperfusion injury (IRI) on the expression of H60 and RAE-1 (MICA, B homologues) in mice to study the protective effect of Yisheng injection (YS), an herbal preparation developed from traditional Chinese medicine.

METHODS:

Male BALB/c mice were divided into sham, ischemic, and YS-treated groups using 90 minutes of left liver lobe ischemia. Sham control mice underwent the same operation, but without vascular occlusion. In the treated group, YS (20 mg/kg) was given before ischemia and after reperfusion for 7 days. Liver samples collected at 7 days postoperation were used for real-time quantitative polymerase chain reaction analysis, Western blotting, and immunohistochemical assays.

RESULTS:

Compared with the sham group, H60 and RAE-1 mRNA levels were increased by sevenfold and 4.5-fold in the ischemic group, respectively. After YS treatment, they were reduced by 76% and 70%, respectively. Western blotting and immunohistochemical assays showed that there was absent or faint H60 and RAE-1 expression in sham liver, but they were apparently increased in ischemic liver; however, the expressions were significantly decreased in the presence of YS.

CONCLUSIONS:

Hepatic IRI significantly increased H60 and RAE-1 expression in mouse liver. YS treatment effectively reduced this increase, seeming to attenuate NKG2D-ligand-mediated immune responses caused by IRI. This may suggest a new concept to prevent IRI and graft rejection.

• Authors: F Cheng, L Feng, S Li, J Tan, L Cao, Y He, Z Ye, Y Li
• Journal: Transplantation proceedings (Transplant Proc) Vol. 38 Issue 7 Pg. 2210-3 (Sep 2006) ISSN: 0041-1345 [Print] United States
• PMID: 16980045 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• Minor Histocompatibility Antigens
• Nuclear Matrix-Associated Proteins
• Nucleocytoplasmic Transport Proteins
• Rae1 protein, mouse
• minor H antigen H60

Topics

• Animals
• Disease Models, Animal
• Drugs, Chinese Herbal (administration & dosage, pharmacology)
• Injections
• Ischemia (genetics)
• Liver Circulation (drug effects)
• Male
• Mice
• Mice, Inbred BALB C
• Minor Histocompatibility Antigens (genetics)
• Nuclear Matrix-Associated Proteins (genetics)
• Nucleocytoplasmic Transport Proteins (genetics)