Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells.

Abstract

The interactions between granulocyte colony-stimulating factor (G-CSF) and dextran sulfate (DS) with different chain lengths and sulfate contents were studied by capillary zone electrophoresis. It was found that DS with a molecular mass of 500 kDa (DS500) could bind to G-CSF and the binding constant and binding sites were determined using Scatchard plot to be 1.17 x 10(6) M(-1) and 3, respectively. DS with a molecular mass of 40 kDa also had the affinity to G-CSF and the binding constant and binding sites were 1.01 x 10(6) M(-1) and 8, respectively. However, DS with a molecular mass of 8 kDa and the non-sulfated saccharide, dextran, had no affinity to G-CSF. The results indicate that the interactions between G-CSF and DS are dependent on the chain lengths and sulfate contents of the saccharides. In addition, the effects of G-CSF-binding DS on a G-CSF-dependent leukemia cell line were investigated using biological methods. Results show that DS500 plus G-CSF has potential therapeutic effect on cancers because their combination could inhibit the growth and induce the differentiation of the leukemia cells.

• Authors: Aiye Liang, Xiaomian Zhou, Qiuyan Wang, Xin Liu, Jianhua Qin, Yuguang Du, Keyi Wang, Bingcheng Lin
• Journal: Electrophoresis (Electrophoresis) Vol. 27 Issue 16 Pg. 3195-201 (Aug 2006) ISSN: 0173-0835 [Print] Germany
• PMID: 16915572 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Granulocyte Colony-Stimulating Factor
• Dextran Sulfate

Topics

• Animals
• Cell Proliferation (drug effects)
• Dextran Sulfate (chemistry, pharmacology, therapeutic use)
• Electrophoresis, Capillary (methods)
• Granulocyte Colony-Stimulating Factor (chemistry, pharmacology, therapeutic use)
• Leukemia (drug therapy)
• Mice
• Molecular Weight
• Protein Binding
• Tumor Cells, Cultured