Abstract | RATIONALE:
Nitric oxide is released from vascular endothelium in response to increased pulsatile shear stress. Nitric oxide inhibits mast cell activation and is antiinflammatory and therefore might be protective in asthma. OBJECTIVES: We determined if a noninvasive motion platform that imparts periodic sinusoidal inertial forces to the whole body along the spinal axis (pGz) causing release of endothelial nitric oxide modulates experimental asthma in sheep. METHODS: RESULTS: pGz treatment for 1 h before antigen challenge reduced the early airway response and blocked the late airway response but did not prevent the antigen-induced airway hyperresponsiveness 24 h after challenge. Administration of L-NAME before pGz completely reversed this protection, whereas L-NAME alone did not affect the antigen-induced responses. NF-kappaB activity was 1.9- and 1.8-fold higher in the control and L-NAME + pGz groups, respectively, compared with pGz-treated animals. Extending the pGz treatment to twice daily for 3 d and then 1 h before antigen challenge blocked the early and late airway responses, the 24-h airway hyperresponsiveness, and the airway inflammatory cell response. CONCLUSION: Whole-body pGz modulates allergen-induced airway responses in allergic sheep.
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Authors | William M Abraham, Ashfaq Ahmed, Irakli Serebriakov, Isabel T Lauredo, Jorge Bassuk, Jose A Adams, Marvin A Sackner |
Journal | American journal of respiratory and critical care medicine
(Am J Respir Crit Care Med)
Vol. 174
Issue 7
Pg. 743-52
(Oct 01 2006)
ISSN: 1073-449X [Print] United States |
PMID | 16858016
(Publication Type: Journal Article)
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Chemical References |
- Antigens, Helminth
- Enzyme Inhibitors
- NF-kappa B
- Nitric Oxide
- NG-Nitroarginine Methyl Ester
|
Topics |
- Acceleration
- Animals
- Antigens, Helminth
- Ascaris suum
(immunology)
- Asthma
(metabolism)
- Disease Models, Animal
- Endothelium, Vascular
(metabolism)
- Enzyme Inhibitors
(pharmacology)
- NF-kappa B
(metabolism)
- NG-Nitroarginine Methyl Ester
(pharmacology)
- Nitric Oxide
(antagonists & inhibitors, metabolism)
- Sheep
- Stress, Mechanical
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