Sendai virus infection up-regulates trypsin I and matrix metalloproteinase-9, triggering viral multiplication and matrix degradation in rat lungs and lung L2 cells.

Abstract

To elucidate the virus-host cell interaction, we analyzed quantitatively the expression of various cellular proteases and tumor necrosis factor-alpha (TNF-alpha) after Sendai virus infection in rat lungs and lung L2 cells. After infection, TNF-alpha mRNA levels increased rapidly to a peak on day one, and then trypsin I and matrix metalloproteinase (MMP)-9, but not MMP-2, were significantly up-regulated with a peak on day 2 in vivo. These up-regulations were confirmed in L2 cells. Up-regulation of proMMP-9 and its active convertase trypsin I seems to synergistically enhance virus multiplication and the destruction of lung matrix, resulting in the progression of pneumonia.

• Authors: H Yamada, Q T Le, A Kousaka, Y Higashi, M Tsukane, H Kido
• Journal: Archives of virology (Arch Virol) Vol. 151 Issue 12 Pg. 2529-37 (Dec 2006) ISSN: 0304-8608 [Print] Austria
• PMID: 16816895 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• Trypsin
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Animals
• Cell Line
• DNA Primers
• Gene Expression Regulation, Enzymologic
• Kinetics
• Lung (virology)
• Matrix Metalloproteinase 2 (genetics)
• Matrix Metalloproteinase 9 (genetics)
• Polymerase Chain Reaction
• Rats
• Sendai virus (pathogenicity, physiology)
• Trypsin (genetics)
• Virus Replication