Abstract | PURPOSE: METHODS: Corneal stromal tissue samples from central and paracentral hypocellular primitive stromal interface scars of human LASIK corneas and from similar regions of normal control corneas were collected by laser capture microdissection (LCM) and subsequently were digested with specific glycosidase enzymes. Digests were directly analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). RESULTS: Concentrations of both monosulfated GlcNAc(6S)-beta-1,3-Gal (MSD2) and disulfated Gal (6S)-beta-1,4-GlcNAc(6S) (DSD) KS disaccharides from the LASIK interface scars were significantly lower than in normal control corneal stromas. No significant difference was found for the concentration of nonsulfated (NSD) KS disaccharides in LASIK interface scars compared with normal controls. The concentration of DeltaUA-beta-1,3-GalNAc(6S) (Deltadi-6S) CS/DS disaccharides from the LASIK interface scar was significantly higher than normal corneal stroma, whereas concentrations of DeltaUA-beta-1,3-GalNAc(4S) (Deltadi-4S) and nonsulfated Deltadi-0S CS/DS disaccharides demonstrated no significant differences from normal corneas. CONCLUSIONS: The profiles of KS and CS/DS disaccharides in LASIK interface scars are significantly different from those in normal cornea stromal tissue, as revealed by LCM and ESI-MS/MS.
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Authors | Yuntao Zhang, Ingo Schmack, Daniel G Dawson, Hans E Grossniklaus, Abigail H Conrad, Yutaka Kariya, Kiyoshi Suzuki, Henry F Edelhauser, Gary W Conrad |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 47
Issue 6
Pg. 2390-6
(Jun 2006)
ISSN: 0146-0404 [Print] United States |
PMID | 16723448
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dermatan Sulfate
- Chondroitin Sulfates
- Keratan Sulfate
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Topics |
- Chondroitin Sulfates
(metabolism)
- Cicatrix
(metabolism)
- Cornea
(surgery)
- Corneal Stroma
(metabolism)
- Dermatan Sulfate
(metabolism)
- Humans
- Keratan Sulfate
(metabolism)
- Keratomileusis, Laser In Situ
- Middle Aged
- Myopia
(surgery)
- Spectrometry, Mass, Electrospray Ionization
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