Investigation of HER-2 codon 655 single nucleotide polymorphism frequency and c-ErbB-2 protein expression alterations in gastric cancer patients.

Abstract

AIM:

To investigate both whether the risk of gastric cancer is associated with the Ile/Val single nucleotide polymorphism (SNP) of human epidermal growth factor receptor-2 (HER-2) transmembrane domain-coding region at codon 655 and the suggested existence of HER-2 expression in gastric cancer cases in a Turkish patient group.

METHODS:

Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) strategy was used to analyze the presence of HER-2 SNP at codon 655. c-erbB-2 expression pattern was analyzed by immunohistochemistry. The results were compared between gastric carcinoma group and chronic gastritis group, as well as between clinicopathological parameters and carcinoma.

RESULTS:

Results showed that Ile/Val genotype accounted for 20% within the Turkish gastric carcinoma group, and none in chronic gastritis group, and this genotyping was associated with stage IV gastric cancers (P=0.04). Positive membranous HER-2 immunoreactivity, on the other hand, accounted for 24% within the Turkish gastric carcinoma group and none from chronic gastritis cases; further, it was correlated with intestinal type carcinomas (P=0.007), and stage III-IV carcinomas (P=0.004).

CONCLUSION:

These observations imply that the tested HER-2 SNP may participate in the development and progression of gastric cancer. Thus, after confirming these results with large sample groups, HER-2 codon 655 SNP and/or c-erbB-2 overexpression may also be used as a poor prognostic indicator for gastric carcinomas.

• Authors: N-Lale Satiroglu-Tufan, Ferda Bir, Nese Calli-Demirkan
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 12 Issue 20 Pg. 3283-7 (May 28 2006) ISSN: 1007-9327 [Print] United States
• PMID: 16718853 (Publication Type: Journal Article)

Chemical References

• Codon
• Receptor, ErbB-2

Topics

• Adult
• Aged
• Aged, 80 and over
• Carcinoma, Adenosquamous (chemistry, genetics)
• Case-Control Studies
• Codon (genetics)
• Female
• Gastritis (genetics)
• Gene Expression Regulation, Neoplastic (genetics)
• Genes, erbB-2 (genetics)
• Genetic Predisposition to Disease
• Humans
• Male
• Middle Aged
• Polymorphism, Single Nucleotide
• Predictive Value of Tests
• Prognosis
• Receptor, ErbB-2 (analysis, genetics)
• Risk Factors
• Stomach Neoplasms (chemistry, genetics)
• Turkey