GAS41 interacts with transcription factor AP-2beta and stimulates AP-2beta-mediated transactivation.
|
| Abstract |
Transcription factor AP-2 regulates transcription of a number of genes involving mammalian development, differentiation and carcinogenesis. Recent studies have shown that interaction partners can modulate the transcriptional activity of AP-2 over the downstream targets. In this study, we reported the identification of GAS41 as an interaction partner of AP-2beta. We documented the interaction both in vivo by co-immunoprecipitation as well as in vitro through glutathione S-transferase (GST) pull-down assays. We also showed that the two proteins are co-localized in the nuclei of mammalian cells. We further mapped the interaction domains between the two proteins to the C-termini of both AP-2beta and GAS41, respectively. Furthermore, we have identified three critical residues of GAS41 that are important for the interaction between the two proteins. In addition, by transient co-expression experiments using reporter containing three AP-2 consensus binding sites in the promoter region, we found that GAS41 stimulates the transcriptional activity of AP-2beta over the reporter. Finally, electrophoretic mobility shift assay (EMSA) suggested that GAS41 enhances the DNA-binding activity of AP-2beta. Our data provide evidence for a novel cellular function of GAS41 as a transcriptional co-activator for AP-2beta.
|
|---|---|
| Authors | Xiaofeng Ding, Changzheng Fan, Jianlin Zhou, Yingli Zhong, Rushi Liu, Kaiqun Ren, Xiang Hu, Chang Luo, Shunyong Xiao, Yeqi Wang, Du Feng, Jian Zhang |
| Journal | Nucleic acids research (Nucleic Acids Res) Vol. 34 Issue 9 Pg. 2570-8 ( 2006) ISSN: 1362-4962 [Electronic] England |
| PMID | 16698963 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!