Abstract | OBJECTIVE: METHODS AND RESULTS:
Apolipoprotein B-48 (apoB-48)-containing lipoprotein metabolism was examined in the steady-state fed condition with a 15-hour primed constant infusion of [D3]- l-leucine in 14 nondiabetic men with a broad range of body mass index (BMI) and insulin sensitivity. To examine the relationship between indices of insulin resistance and intestinal lipoprotein PR data were analyzed in 2 ways: by correlation and by comparing apoB-48 PRs in those whose fasting plasma insulin concentrations were above or below the median for the 14 subjects studied (60 pmol/L). ApoB-48 PR was significantly higher in hyperinsulinemic, insulin-resistant subjects (1.73+/-0.39 versus 0.88+/-0.13 mg/kg per day; P<0.05) and correlated with fasting plasma insulin concentrations (r=0.558; P=0.038), despite great heterogeneity in apoB-48 kinetic parameters, particularly among the obese subjects. There was no significant difference in clearance of apoB-48 between the 2 groups, nor was there a significant correlation between apoB-48 fractional clearance rate and fasting insulin or homeostasis model assessment- insulin resistance. CONCLUSIONS: These are the first human data to conclusively demonstrate that intestinal apoB-48-containing triglyceride-rich lipoprotein PR is increased in hyperinsulinemic, insulin-resistant humans. Intestinal lipoprotein particle overproduction is a newly described feature of insulin resistance in humans.
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Authors | Hélène Duez, Benoît Lamarche, Kristine D Uffelman, René Valero, Jeffrey S Cohn, Gary F Lewis |
Journal | Arteriosclerosis, thrombosis, and vascular biology
(Arterioscler Thromb Vasc Biol)
Vol. 26
Issue 6
Pg. 1357-63
(Jun 2006)
ISSN: 1524-4636 [Electronic] United States |
PMID | 16614317
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoprotein B-48
- Apolipoproteins
- Apolipoproteins B
- Insulin
- Lipids
- Lipoproteins
- Triglycerides
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Topics |
- Adult
- Apolipoprotein B-48
- Apolipoproteins
(blood)
- Apolipoproteins B
(analysis, biosynthesis, blood)
- Eating
- Fasting
(blood)
- Homeostasis
- Humans
- Hyperinsulinism
(metabolism, physiopathology)
- Insulin
(blood)
- Insulin Resistance
- Intestinal Mucosa
(metabolism)
- Lipids
(blood)
- Lipoproteins
(biosynthesis, blood, chemistry)
- Male
- Middle Aged
- Osmolar Concentration
- Triglycerides
(blood)
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