A simplified method for immunological typing of trachoma-inclusion conjunctivitis-lymphogranuloma venereum organisms.

Abstract

Responding to the pressure of large numbers of trachoma-inclusion conjunctivitis (TRIC)-lymphogranuloma venereum (LGV) isolates from field studies requiring serotyping, we have developed a simplified, less-precise method that utilizes cell culture-grown organisms to produce mouse antisera which is tested agaist prototype TRIC-LGV antigens in the micro-immunofluorescence test. Cell cultures with as few as 5 to 15% of cells showing inclusions produced adequate antibody in mice 4 days after single injection. Knowledge of the reaction of prototype antisera with the antigens has allowed typing of most isolates tested from the pattern of cross-reaction of their antiserum.

• Authors: S P Wang, C C Kuo, J T Grayston
• Journal: Infection and immunity (Infect Immun) Vol. 7 Issue 3 Pg. 356-60 (Mar 1973) ISSN: 0019-9567 [Print] United States
• PMID: 16558075 (Publication Type: Journal Article)