Abstract | BACKGROUND: It was previously reported that a functional human (h) Rad9 protein accumulated in the nuclei of non-small cell lung carcinoma (NSCLC) cells. Those experiments, however, did not examine whether the hRad9 gene was mutated in those cells. The sequence of the HRAD9 gene in NSCLC cells was investigated. METHODS: The sequence of the HRAD9 was examined in tumor and peripheral normal lung tissues obtained from 50 lung adenocarcinoma patients during surgery. The expression of its mRNA using reverse transcription polymerase chain reaction (RT-PCR) was also examined. RESULTS: No sequence alterations were detected in the HRAD9 gene, which was found to be normally transcribed in surgically resected lung carcinoma cells. However, in eight (16.0%) cases a single nucleotide polymorphism (SNP) was observed at the second position of codon 239 (His/Arg heterozygous variant) of the gene. This frequency was significantly higher than that found in the normal population. CONCLUSIONS:
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Authors | Yoshimasa Maniwa, Masahiro Yoshimura, Vladimir P Bermudez, Kenji Okada, Naoki Kanomata, Chiho Ohbayashi, Yoshihiro Nishimura, Yoshitake Hayashi, Jerard Hurwitz, Yutaka Okita |
Journal | Cancer
(Cancer)
Vol. 106
Issue 5
Pg. 1117-22
(Mar 01 2006)
ISSN: 0008-543X [Print] United States |
PMID | 16444745
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cell Cycle Proteins
- rad9 protein
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Topics |
- Adenocarcinoma
(genetics)
- Carcinoma, Non-Small-Cell Lung
(genetics)
- Cell Cycle Proteins
(genetics)
- DNA Mutational Analysis
- Humans
- Lung Neoplasms
(genetics)
- Polymorphism, Single Nucleotide
- Reverse Transcriptase Polymerase Chain Reaction
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