Abstract | BACKGROUND: Wheat is involved in different forms of respiratory, food and contact allergy. The IgE of patients generally reacts with various flour proteins. It is not known if antigenic relationships could explain some of these reactions and if proteins could be involved in different pathologies. METHODS: RESULTS: The IgE from both sera strongly reacted with natural and recombinant gamma-3 hordein but displayed different patterns of reactivity with wheat, barley and rye proteins. Those from the WDEIA patient showed expected reactions with omega-5 gliadin, gamma-35 and gamma-75 secalins, but also with wheat low-molecular-weight glutenin subunits (LMW-GS), and not with C hordeins. On the contrary, IgE from a HHWP patient reacted with C hordeins, various omega gliadins, and gamma-75 secalin, but very weakly with gamma-35 secalin and LMW-GS. Recombinant gamma-3 hordein inhibited strongly but not totally the WDEIA patient's IgE binding to prolamins. No such inhibition could be observed for the HHWP patient's IgE. CONCLUSIONS: At least part of the reactions of prolamins with the IgE from the WDEIA patient was due to antigenic homologies. The occurrence of cross-reacting carbohydrates was unlikely. These common IgE epitopes were not involved in the pathology of the HHWP patient.
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Authors | Jacques Snégaroff, Isabelle Bouchez-Mahiout, Catherine Pecquet, Gérard Branlard, Michel Laurière |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 139
Issue 3
Pg. 201-8
( 2006)
ISSN: 1018-2438 [Print] Switzerland |
PMID | 16439858
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright (c) 2006 S. Karger AG, Basel. |
Chemical References |
- DNA, Plant
- Epitopes
- Plant Proteins
- Recombinant Proteins
- Immunoglobulin E
- Glutens
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Topics |
- Adult
- Amino Acid Sequence
- Anaphylaxis
(etiology, immunology)
- Cross Reactions
- DNA, Plant
(chemistry, genetics)
- Epitopes
- Exercise
- Female
- Glutens
- Hordeum
(immunology)
- Humans
- Immunoblotting
- Immunoglobulin E
(immunology)
- Middle Aged
- Molecular Sequence Data
- Plant Proteins
(chemistry, genetics, immunology)
- Polymerase Chain Reaction
- Recombinant Proteins
(genetics, immunology)
- Secale
(immunology)
- Sequence Homology, Amino Acid
- Wheat Hypersensitivity
(immunology)
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