Abstract | UNLABELLED: The relative importance of endothelium- and cardiomyocyte-derived nitric oxide (NO) is unknown, with a lack of direct studies on cardiac microvessel endothelial cells ( CMEC) and cardiomyocytes regarding relative cellular NO production. AIMS: To assess and compare baseline and hypoxia-induced NO and ONOO- production in cardiomyocytes and CMEC. METHODS: Rat cardiomyocytes were isolated, and cultured rat CMEC were purchased commercially. Hypoxia (+/- NOS inhibitors) was induced by mineral oil layering or hypoxic culture. NO and ONOO- were detected by FACS analysis of DAF-2/DA and DHR123, respectively. Total eNOS was determined by Western blot analysis. RESULTS: 1) Baseline NO production in CMEC was sevenfold (cultured cells) and 26-fold (isolated cells) higher than in cardiomyocytes, 2) eNOS expression was 22-fold higher in CMEC, 3) hypoxia increased NO production in both cell types, albeit to a larger extent in CMEC, 4) in hypoxic cardiomyocytes, nonselective NOS and iNOS-specific inhibition attenuated NO production, whereas in CMEC, iNOS-specific inhibition was ineffective, and 5) baseline ONOO- production was 2.2 times greater in CMEC than in cardiomyocytes. CONCLUSION: Using a novel approach, this study demonstrated that CMEC produce more baseline NO than cardiomyocytes, and that hypoxia activates NOS to increase NO production in both cell types. Baseline eNOS content was higher in CMEC than in cardiomyocytes, suggesting that differences in baseline NO production were eNOS-associated.
|
Authors | Hans Strijdom, Sean Jacobs, Suzél Hattingh, Carine Page, Amanda Lochner |
Journal | FASEB journal : official publication of the Federation of American Societies for Experimental Biology
(FASEB J)
Vol. 20
Issue 2
Pg. 314-6
(Feb 2006)
ISSN: 1530-6860 [Electronic] United States |
PMID | 16354719
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Peroxynitrous Acid
- Nitric Oxide
- Nitric Oxide Synthase Type III
|
Topics |
- Animals
- Coronary Vessels
(cytology)
- Endothelial Cells
(metabolism)
- Hypoxia
(metabolism)
- Male
- Myocytes, Cardiac
(metabolism)
- Nitric Oxide
(biosynthesis, metabolism)
- Nitric Oxide Synthase Type III
(metabolism)
- Peroxynitrous Acid
(metabolism)
- Rats
- Rats, Wistar
|