Abstract | PURPOSE: METHODS: PA was incorporated in LPD particles and nasally dosed to mice. The resulting PA-specific immune response and lethal toxin neutralization activity were measured. RESULTS: Mice nasally immunized with PA incorporated into LPD particles developed both systemic and mucosal anti-PA responses. The anti-PA immunities induced included the production of anti-PA antibodies ( IgG and IgM in the serum and IgA in nasal and lung mucosal secretions) and the proliferation of splenocytes after in vitro stimulation. The anti-PA IgG subtype induced was mainly IgG1. Finally, anthrax lethal toxin neutralization activity was detected both in the serum and in the mucosal secretions. CONCLUSIONS: The anti-PA immune response induced by nasal PA incorporated in LPD was comparable to that induced by nasal PA adjuvanted with cholera toxin or subcutaneously injected PA adjuvanted with aluminum hydroxide.
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Authors | Brian R Sloat, Zhengrong Cui |
Journal | Pharmaceutical research
(Pharm Res)
Vol. 23
Issue 2
Pg. 262-9
(Feb 2006)
ISSN: 0724-8741 [Print] United States |
PMID | 16319999
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anthrax Vaccines
- Antibodies, Bacterial
- Bacterial Toxins
- Immunoglobulin A
- Liposomes
- Protamines
- Vaccines, DNA
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Topics |
- Administration, Intranasal
- Animals
- Anthrax Vaccines
(administration & dosage, immunology, pharmacokinetics)
- Antibodies, Bacterial
(analysis, biosynthesis)
- Bacterial Toxins
(antagonists & inhibitors, toxicity)
- Cell Proliferation
(drug effects)
- Enzyme-Linked Immunosorbent Assay
- Female
- Immunoglobulin A
(biosynthesis)
- Liposomes
- Mice
- Mice, Inbred BALB C
- Neutralization Tests
- Protamines
- Spleen
(cytology, drug effects)
- Vaccines, DNA
(administration & dosage, immunology, pharmacokinetics)
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