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Anti-SARS-CoV immunity induced by a novel CpG oligodeoxynucleotide.

Abstract
To develop CpG oligodeoxynucleotides (CpG ODNs) based therapy for prevention and treatment of severe acute respiratory syndrome (SARS), we selected a novel CpG ODN (BW001), which displays B-type CpG ODN structure feature at the 5' and A-type CpG ODN structure feature at the 3', and tested for its anti-SARS-CoV activity. We found that the supernatants of human PBMCs stimulated by BW001 significantly protected Vero cells from SARS-CoV infection. BW001 could stimulate human PBMCs and pDCs to secrete high level of IFN-alpha and promote human PBMCs and B cells to proliferate. Furthermore, we demonstrated that BW001 could activate CD19+ B cells and CD56+ NK cells in human PBMCs. In addition, BW001 could enhance NK cytotoxicity and IFN-gamma secretion in human PBMCs. Together, BW001 represents a novel type of CpG ODN and may have potential for the development of treatment and prevention for SARS as well as other viral associated diseases.
AuthorsMusheng Bao, Yi Zhang, Min Wan, Li Dai, Xiaoping Hu, Xiuli Wu, Li Wang, Ping Deng, Junzhi Wang, Jianzhu Chen, Yongjun Liu, Yongli Yu, Liying Wang
JournalClinical immunology (Orlando, Fla.) (Clin Immunol) 2006 Feb-Mar Vol. 118 Issue 2-3 Pg. 180-7 ISSN: 1521-6616 [Print] United States
PMID16298165 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adjuvants, Immunologic
  • Antiviral Agents
  • CPG-oligonucleotide
  • Oligodeoxyribonucleotides
Topics
  • Adjuvants, Immunologic (pharmacology)
  • Animals
  • Antiviral Agents (pharmacology)
  • B-Lymphocytes (immunology, virology)
  • Cell Line
  • Chlorocebus aethiops
  • CpG Islands (immunology)
  • Cytotoxicity Tests, Immunologic
  • Humans
  • Killer Cells, Natural (immunology, virology)
  • Leukocytes, Mononuclear (immunology, virology)
  • Lymphocyte Activation (immunology)
  • Oligodeoxyribonucleotides (immunology)
  • Severe acute respiratory syndrome-related coronavirus (immunology)
  • Severe Acute Respiratory Syndrome (immunology, prevention & control)
  • Vero Cells

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